Background: background: Indonesia is one of the countries with a wealth of flora in the world, has a variety of plants that have the potential for cancer treatment, one of which is the Senduduk Leaf plant (Melastoma malabathricum) or MM. Previous researchers reported that the methanol extract from the leaves showed significant anticancer activity against the MCF-7 cell line with an IC50 of 7.14 g/ml. This research is a follow-up analysis of previous research which aims to examine cytotoxic activity and identify plant chemical compounds used for traditional cancer treatment in Indonesia. Materials and Methods: The materials used in this study were MM leaf simplicia, 96% ethanol, test tube reagents, MCF-7 cells, RPMI culture media, DMSO, MTT reagents, PBS, SDS. The extraction method used was maceration, phytochemical screening analysis using test tube reagents and TLC profile confirmation along with cytotoxic activity testing using the MTT Assay method.........

Keywords:- Melastoma malabathricum, Cytotoxic, MCF-7 Cells, MTT Assay.

[1]. World Health Organization, 2013. WHO handbook for reporting results of cancer treatment. World Health Organization.
[2]. Zahreddine, H. and Borden, KL, 2013. Mechanisms and insights into drug resistance in cancer. Frontiers in pharmacology, 4, p.28.
[3]. Idris, A., Zulkipli, IN, Zulhilmi, NR, Lee, HF, Rajabalaya, R., Chee, LY, Majid, M. and David, SR, 2017. Melastoma malabathricum ethyl acetate fractions induces secondary necrosis in human breast and lung cancer cell lines. Pharmacognosy Magazine, 13(Suppl 3), p.S688.
[4]. Ali, DMH, Keng, CW and Boey, PL, 2010. Triterpenoids, glycolipids and flavonoids of Melastoma malabathricum: Isolation, spectrometric characterization and antibacterial activity. VDM Publishing.
[5]. Salleh, MM, Eshak, Z. and Ismail, WW, 2017. Acacia honey induces apoptosis in human breast adenocarcinoma cell lines (MCF-7). J. Technol, 79, pp.9-16.

Background: Satisfying patients with pharmaceutical care has evolved as a requirement for improving patients' health behaviors, leading to better health care outcomes. Aim: The study's goal was to analyze outpatient satisfaction and discover predictors of how satisfied patients are with the provision of pharmaceutical care in a public secondary health institution. Methods: Using the method of convenient sampling, a sample of 400 patients from the health facility's outpatient department was enlisted. 400 questionnaires were distributed, and 380 were returned. Results: The weighted mean of the responses showed that respondents were extremely delighted with the corporate look of the pharmacy.........

Keywords:- patient's, pharmaceutical care, Pharmacist, Secondary Healthcare, satisfaction

[1]. Accreditation Council for Pharmacy Education - ACPE (2011). Accreditation Standards and Guidelines for the Professional Program in Pharmacy Leading to the Doctor of Pharmacy Degree. Chicago, Illinois, 2011. [cited 2014, Sep11] available from: https://www.acpe accredit.org/standards/.
[2]. Ala'Eddin MA, Mohammad AS, Saleh AS, Omar ZA, Hussein MA (2016). Factors Influencing Patient Satisfaction with Pharmacy Services: An Empirical Investigation at King Fahd Armed Forces Hospital, Saudi Arabia. International Journal of Business and Management 11(9).
[3]. Arneson DL, Jacobs EW, Scott DM (1989). Patronage motives of community pharmacy patrons. J Pharm Mark Manage; 4(2):3-22.
[4]. Azuka C. Opara, Ehijie FO Enato, Obehi Akoria (2004). Assessment of patient satisfaction with pharmaceutical services in a Nigerian Teaching Hospital. International Journal of Pharmacy Practice; 12(1): 7-12. https://doi.org/10.1211/0022357023204
[5]. Barbosa CD, Balp MM, Kulich K, Germain N, Rofail D (2012). A literature review to explore the link between treatment satisfaction and adherence, compliance, and persistence. Patient Prefer Adherence; 6: 39–48. 10.2147/PPA.S24752.

At the heart of any mass spectrometer is the mass analyzer. This component takes the ionized masses and separates them based on their mass-to-charge ratio (m/z). Mass analyzers are instrumental components in analytical chemistry, biochemistry, and various scientific disciplines that require precise and accurate determination of molecular masses. After samples are ionized in a source box, the mass analyzer separates the ions according to their m/z value. The most common mass spectrometer is the quadrupole (QP) type, which is ideal as a practical and inexpensive instrument because of its compactness, easy operation, easy maintenance, high sensitivity, fast scan rate, and moderate vacuum requirement. The mass spectrometer takes ionized masses and separates them based on mass-to-charge ratios.........

Keywords:- mass analyzers, time of flight, quadrupole, ion trap

[1]. El-Aneed A, Cohen A, Banoub J. Mass spectrometry, review of the basics: electrospray, MALDI, and commonly used mass analyzers. Applied spectroscopy reviews. 2009 Apr 1;44(3):210-30.
[2]. Brunnée C. The ideal mass analyzer: fact or fiction? International journal of mass spectrometry and ion processes. 1987 Jun 1;76(2):125-237.
[3]. Naseri A, Johnson TJ, Smallwood G, Olfert JS. Measurement of the centrifugal particle mass analyzer transfer function. Aerosol Science and Technology. 2023 Jun 22: 1-20.
[4]. Karlsson A, Lodeng R, Haugholt KH, Myhrvold E, Plassen M, Thorshaug K. High-performance fixed-bed in situ mass analyzer—ISMA. Review of Scientific Instruments. 2023 Jun 1;94(6).
[5]. Chantipmanee N, Xu Y. Toward nanofluidic‐based mass spectrometry for exploring the unknown complex and heterogeneous subcellular worlds. View. 2023 Feb;4(1):20220036.