Background: Bioanalysis plays a crucial role in the processes of drug development and drug discovery. It serves as an essential
component of toxicological evaluations, as well as pharmacokinetic and pharmacodynamic studies during the development of drugs. The bioanalysis procedure involves several stages, including sampling, sample preparation, analysis, calibration, and data evaluation and reporting. However, the development of bioanalytical methods presents a significant bottleneck in drug development. Furthermore, the validation of these methods is vital for accurately quantifying various analytes in biological samples. Method validation ensures the acceptability of assay performance and.........

Keywords:- Bioanalysis, Bioanalytical method development, validation, Drug development, Drug discovery.

[1]. Dighe S, Shah VP, Midha KK, McGilveray IJ, Skelly JP, Yacobi A,etal,Analytical methods validation: bioavailability, bioequivalence and pharmacokinetics studies, Conference Report, Eur J Drug Metabol Pharmacokinetics, 16, 1998, 249–55.
[2]. Shah VP, Midha KK, Dighe S, McGilveray IJ, Skelly JP, Yacobi A, et al. Analytical methods validation: bioavailability, bioequivalence and pharmacokinetic studies. Conference report. Pharm Res. 2009;9:588–92.
[3]. Causon R. Validation of chromatographic methods in biomedical analysis viewpoint and discussion. J Chromatogr. 1998;689:175–80.
[4]. Raymond NX, Fan LR, Matthew J, Tawakol A. Recent advances in high-throughput quantitative bioanalysis by LC–MS/MS. J Pharm Biomed Anal. 2007;44:342–55.
[5]. P. Ravi Sankar, A. Sai Geethika, G. Rachana, P. Srinivasa Babu, J. Bhargav, Bioanalytical Method Validation: A Comprehensive Review, Int. J. Pharm. Sci. Rev. Res.,2019, 56(1), 50-58.-7). J. Technol, 79, pp.9-16.

Background: the study aimed to analyze the perceived stress level, the fear of accessing health care (i.e., from the pharmacy, hospital, general practitioner's office, dental office), and drug uptake during and after the COVID-19 lockdown. Methods: A prospective study was conducted on a convenience sample. There were included persons 18 years of age or older living in Bucharest. Data were collected through questionnaires administered by phone interviews during the COVID-19 lockdown (T1-May 2020), and four months after it (T2- September 2020). Results: Fifty-three participants were included. The stress level was statistically significantly higher in T1 than in T2 (p=0.011). Fear.......

Keywords:-stress, SARS-CoV-2, self-medication, medicine, drugs

[1]. Adam M, Urbancic-Rak T, Crnic T. Dental students' discomfort and anxiety during the first and the second lockdown due to COVID-19 pandemic at the School of Dental Medicine, University of Zagreb. Acta Stomatol. Croat. 2021;55(2): 186–197.
[2]. Alimoradi Z, Ohayon MM, Griffiths MD, Lin CY, Pakpour AH. Fear of COVID-19 and its association with mental health-related factors: systematic review and meta-analysis. BJPsych open. 2022;8(2): e73.
[3]. Al-Rawi BA, Alobaid AM, Al-Wathinani AM, Abdulaziz A, Alameen I, Syed W. Evaluation of knowledge on the preventive practice of COVID-19 among middle-aged adults in Saudi Arabia a cross-sectional questionnaire based study. Farmacia. 2022;70(5): 891–896.
[4]. Armfield J. M. Development and psychometric evaluation of the Index of Dental Anxiety and Fear (IDAF- 4C+). Psychol Assess. 2010;22(2): 279–287.
[5]. Bicheru M, Preoteasa CT, Zamfirescu A, Capisizu A, Melescanu Imre M, Preoteasa E. Relation of polypharmacy to systemic and oral health related factors. Rev. Chim. 2018;69(11): 3115–3117.

The technology sector has undergone a significant industrial revolution as a result of the scientific understanding and practical application of artificial intelligence and (3D) printing, which were previously unimaginable. Applications can be found in a variety of field, including aerospace, architecture industry, automobile industry, engineering, chemical industry, military, fashion industry, dental and in healthcare. Particularly in the pharmaceutical industry, the 3DP approach has so far proven to be one of the most inventive technologies [1]. The International Standard Organisation (ISO) defines 3DP as "fabrication of objects through the deposition of a material using a print head, nozzle, or other printer technology." Three-dimensional printing is one of the methods for fabricating additive layers [3]. The internal 3D printing procedure entails two steps: (i) Direct software data transfer to printed structures; and.......

Keywords:-....

[1]. Islam R, Sadhukhan P. An insight of 3D printing technology in pharmaceutical development and application: an updated review. Current Trends in Pharmaceutical Research. 2020;7(2):55-80.
[2]. Samiei N. Recent trends on applications of 3D printing technology on the design and manufacture of pharmaceutical oral formulation: a mini review. Beni-Suef University Journal of Basic and Applied Sciences. 2020;9(12):1-12. https://doi.org/10.1186/s43088-020-00040- 4.
[3]. Jamroz W, Szafraniec J, Kurek M, Jachowicz R. 3D Printing in Pharmaceutical and MedicalApplications – Recent Achievements and Challenges. Pharmaceutical research. 2018;35(176):1-22. https://doi.org/10.1007/s11095-018-2454-x.
[4]. Gujrati A, Sharma A, Mahajan SC. Review on Applications of 3D Printing in Pharmaceuticals. International Journal of Pharmaceutical Sciences Review and Research.2019; 59(1):148-154.
[5]. Ramya A, Vanapalli SL. 3D printing technologies in various applications. International Journal of Mechanical Engineering and Technology (IJMET). 2016;7(3):396–409.

BACKGROUND: Breast cancer is an uncontrolled growth of epithelial cells that can be originating in ducts or lobes in the breast. Among the chemotherapy, Adriamycin, Cyclophosphamide, Trastuzumab, Tamoxifen, Carboplatin, Paclitaxel, Docetaxel, Letrozole, Anastrozole, etc are used to treat breast cancer. MATERIALS AND METHODS: Our study was a Prospective Observational that was carried out at Omega Hospitals, Guntur. Data was collected by the patient or the patient representative or by studying the patient's profile through a questionnaire. The patient proforma included key information on the patient's demographics, past medical history, diagnostic tests, treatment, 2D Echo, safety profile, Mental health assessment, and quality of life. RESULTS: The collected data was transported to SPSS (version-28) for analysis. Out of 84 patients, 9 patients were seen with trastuzumab-induced cardiotoxicity. Of those 8 patients had reversible cardiotoxicity (88.88%) after ACE treatment and 1......

Keywords:- Trastuzumab, cardiotoxicity, mental health assessment, quality of life.

[1]. World Health Organization. Cancer. [Internet]. World Health Organization; 2021 [cited 2021 Sep 28].
[2]. Slamon DJ, C. G. (1987). 2. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 1987;235(4785):177-182.
[3]. Cancer., W. H. (2021). World Health Organization. Cancer. [Internet]. World Health Organization; 2021 [cited 2021 Sep 28].
[4]. Slamon DJ, E. W. (2011). Adjuvant trastuzumab in HER2-positive breast cancer. N Engl J Med. 2011;365(14):1273-1283.
[5]. Baselga J, C. J. (2012). Pertuzumab plus trastuzumab plus docetaxel for metastatic breast cancer. N Engl J Med. 2012;366(2):109-119..

Background: Poison is a substance that causes injury or illness or death, even when taken in a little quantity. Poisoning isa significant global public health problem ranking 45th in total deaths worldwide. Every year half a million people die due to poisoning. The main aim of the study is to assess the incidence and treatment patterns in intentional poisoning patients. Materials and Methods: A prospective observational study for six months duration was conducted at secondary care hospital on 196 cases of Intentional poisoning reported to emergency department from Dec 2021 to May 2022,.regarding patient demographics, type of poisoning ,and its treatment were collected and analyzed using Microsoft excel 2010........

Keywords:-Intentional poisoning, Antidote, Glasgow coma scale, Drug overdose, secondary care hospital.

[1]. Hospital corpsman 3 & 2- intro navy nursing manual for hospital training purposes, available at. Medical. Tpud.com/10669-css/10669- c_279-.284htm.
[2]. World Health Organization. Poisoning [document on the internet]. 2010 [cited 2010 Oct 30]. Available from: http://www.who.int/topics/poisons/en.
[3]. Dash SK, Raju AS, Mohanty MK, Patnaika KK, Siddharatha P, Mohanty S. Sociodemographic Profile of Poisoning Cases. JIAFM. 2005; 27: 133-8.
[4]. Batra AK, Keoliya AN, Jadhav GU. Poisoning: An Unnatural cause of morbidity and mortality in Rural India. JAP. 2003;51:955-960.
[5]. Available from. http://www.kppub.com/articles/march2009/need_ for_poison_information_centres.html.