Regenerative endodontics is an innovative field focused on biologically revitalizing necrotic teeth, aiming to restore the pulp-dentin complex and promote root development. This approach contrasts with traditional root canal therapy by leveraging tissue engineering, stem cell therapy, and molecular biology to extend tooth vitality, particularly benefiting young patients with immature permanent teeth. Regenerative endodontics has emerged as a revolutionary field in dental medicine, aiming to replace damaged structures, including dentin, root, and cells of the pulp-dentin complex. This is achieved through the utilization of stem cells, growth factors, and scaffolds, which collectively facilitate the regeneration of functional dental tissues. Recent advancements have significantly enhanced the outcomes and broadened the scope of regenerative endodontic procedures (REPs). These advancements have important implications for prosthodontics, offering new opportunities to preserve natural teeth and enhance prosthetic outcomes..........

[1]. Ruparel, N. B., Teixeira, F. B., Ferraz, C. C., & Diogenes, A. (2012). Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. *Journal of Endodontics, 38*(10), 1372-1375.
[2]. Banchs, F., & Trope, M. (2004). Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? *Journal of Endodontics, 30*(4), 196-200.
[3]. Hoshino, E., Kurihara-Ando, N., Sato, I., Uematsu, H., Sato, M., Kota, K., ... & Kakehashi, S. (1996). In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. *International Endodontic Journal, 29*(2), 125-130.
[4]. Diogenes, A., Henry, M. A., Teixeira, F. B., & Hargreaves, K. M. (2016). An update on clinical regenerative endodontics. *Endodontic Topics, 28*(1), 2-23.
[5]. Nagata, J. Y., Gomes, B. P. F. A., Rocha Lima, T. F., Murakami, L. S., de Faria, D. E., & Uchoa, A. F. (2014). Phytic acid for root canal irrigation: effects on smear layer and microhardness. *Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 117*(6), e153-e158.

This abstract provides an overview of the comprehensive guide on HPLC method optimization, development, and validation, focusing on insights into chromatographic parameter variability. It emphasizes the importance of HPLC in ensuring the quality, safety, and efficacy of medications through pharmaceutical analysis. The abstract highlights the principles of separation underlying HPLC, the role of instrumentation in enhancing selectivity, and the systematic approach to method development. Additionally, it discusses the guidelines for analytical method validation to ensure the accuracy and reliability of HPLC methods. The abstract concludes by addressing the impact of chromatographic parameter variability on method performance and the importance of managing these factors for consistent and reliable results in pharmaceutical analysis.

KEYWORDS: HPLC method optimization, Pharmaceutical analysis, Chromatographic parameter variability, Method development, Analytical method validation.

[1]. Nikolin B, Imamović B, Medanhodzić-Vuk S, Sober M. High perfomance liquid chromatography in pharmaceutical analyses. Bosn J Basic Med Sci. 2004;4(2):5-9.
[2]. Meyer, V. R. (2009). Praxis der Hochleistungs-Flüssigchromatographie. John Wiley & Sons.
[3]. P. Ravi Sankar, A. Sai Geethika, G. Rachana, P. Srinivasa Babu, J. Bhargav, Bioanalytical Method Validation: A Comprehensive Review, Int. J. Pharm. Sci. Rev. Res.,2019, 56(1), 50-58.
[4]. Lavanya Chowdary G, Ravisankar P, Akhil Kumar G, Mounika K, Srinivasa Babu, Analytical method validation parameters: An updated review. Int. J. Pharm. Sci. Rev.Res., 2020;61(2):1-7.
[5]. Ravisankar P, Naga Navya Ch, Pravallika D, Navya Sri D. A review on step-by-step analytical method validation. IOSR J Pharm. 2015;5(10):7-19.

Research done in the last five decades showed the applicability and advantage of instrumental color measurement to many areas in the pharmaceutical field where the determination of appearance is relevant, including the development, production and quality control of API, excipients and finished pharmaceutical products. The development of instrumental color measurement applications in the pharmaceutical sector, in recent years led to a significant degree of harmonization through the major pharmacopoeias,United States Pharmacopoeia (USP), European Pharmacopoeia (Ph. Eur.) and Japanese Pharmacopoeia(JP). Still, instrumental color measurement has not become a routine method in the quality control of medicines, as is visual color determination. In the measurement of color.......

KEYWORDS: Instrumental color measurement, manual calculation, pharmaceutical routine quality control, spectrophotometric color measurement, tristimulus colorimetry.

[1]. Mettler Toledo 2024, Mettler Toledo website, accessed 17August 2024, <https://www.mt.com/ca/en/home/applications/Application_Browse_Laboratory_Analytics/uv-vis-spectroscopy/uv-vis-color-measurement.html>.
[2]. Šubert, J., Čižmárik, J., & Kolář, J. (2020). Advances in the use of instrumental measurement of colour in the development, production and quality control of drugs, medicinal preparations and pharmaceutical auxiliary substances III. Ceska Slov Farm., 69(2), 59-66.
[3]. Šubert, J., & Čižmárik, J. (2014). Advances in the use of instrumental measurement of colour in the development, production and quality control of drugs, medicinal preparations and pharmaceutical auxiliary substances II. Ceska Slov Farm., 63(3), 107-12.
[4]. Šubert, J., & Čižmárik, J. (2013). Advances in the use of instrumental measurement of colour in the development, production and quality control of drugs, medicinal preparations and auxiliary substances I. Čes. slov. Farm., 62, 65–70.
[5]. Šubert, J., & Čižmárik, J. (2008). Application of instrumental colour measurement in development and quality control of drugs and pharmaceutical excipients. Die Pharmazie, 63(5), 331-336.

Pharmacovigilance (PV) plays a key role in the healthcare system through assessment, monitoring & discovery of interactions amongst drugs and their effects in human being. Pharmaceutical and biotechnological products are designed to diagnose, prevent or cure diseases. India is the world's second most populated country with over 1 billion potential drug consumers. Although, India is participating in the Uppsala Monitoring Center (UMC) programme, its contribution to that database is relatively small. Signal assessment is mainly performed to analyse the cause and effect by using World Health Organization (WHO) scale & Naranjo scale of probability. Signal detection and its assessment is very vital and complex process. This article gives a systematic review of the PV In India from its origin to the current scenario and also discusses the various strategies and proposals to build, maintain and implement a robust PV system and to improve the process of ADR reporting in the country.

KEYWORDS: Adverse drug reactions; pharmacovigilance; spontaneous reporting system; Uppsala monitoring centre; New updatez,causality assessment system.

[1]. WHO. The Importance of Pharmacovigilance: Safety Monitoring of medicinal products. 2002;
[2]. Singh KNM and Kanase HR. Pharmacovigilance Programme of India: The Beginning, Current Status and Recent Progress. Adv Pharmacoepidemiol Drug Saf. 2017;6(4):1-4. Doi: 10.4172/2167- 1052.1000219
[3]. Pharmacovigilance. World Health Organization. 2017.
[4]. Kalaiselvan V, Thota P, Singh GN. Pharmacovigilance Programme of India: Recent developments and future perspectives. Indian J Pharmacol. 2016;48(6):624-628. Doi: 10.4103/0253-7613.194855.
[5]. PvPI Reaches out to rural masses. Newsletter Pharmacovigilance Programme of India. 2017.