Paper Type	::	Research Paper
Title	::	Correlation Of Body Composition With Dynamic Lung Function.
Country	::	India
Authors	::	Dr.SwikrutiBehera||Dr.Bipinbihari pradhan
Page No.	::	01-03
Paper Index	::	DOI : 10.9790/3013-312013
ANED	::	DOI :05.3013/03120103

Objectives: Abstract:Lung volumes that depend upon the rate at which air flows out of the lungs are termed dynamic lung volume. Dynamic lung volumes include Forced Vital Capacity(FVC), forced Expiratory Volume in 1 Second(FEV1)& FEV1/FVC ratio. These lung parameters are very commonly used in clinical practice to diagnose various obstructive and restrictive diseases. For long, Body Mass Index & Waist Hip Ratio are being used to assess the obesity and their effect on lung function. But BMI has the limitation of not distinguishing between fat and muscle mass. So this study was undertaken to study that which body parameter is responsible for improved or worsened lung function.150 healthy medical students(85 males and 65 females) with age group 18-24 years were included in the study. Their body composition was studied using 'Bioelectric Impedance' method. Each dynamic lung function was correlated with body fat percentage (BF%), fat free mass(FFM), fat free mass index(FFMI), BMI & waist hip ratio. FFM showed highest as well as significant correlation with FVC, FEV1& FEV1/FVC.BF% has insignificant correlation with all dynamic lung function. BMI & W/H ratio, have lower or insignificant correlation with dynamic lung functions, than FFM & FFMI.

 

Keywords: Dynamic lung volumes, Forced vital capacity, Forced expiratory volume, Body mass index, Waist hip ratio, Body fat percentage, Fat free mass, Fat free mass index.

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Paper Type	::	Research Paper
Title	::	Resveratrol And Its Derivative Improves Oxidative Stress and Protects Against Alloxan- Induced Diabetics
Country	::	Iraq
Authors	::	Montaha A. Al-Safar
Page No.	::	.04-09
Paper Index	::	DOI : 10.9790/3013-312049
ANED	::	DOI : 05.3013/03120409

Objectives: Resveratrol is a non flavonoid polyphenol and it has three phenolic hydroxyl groups and shown to have its biological effects . Reactive oxygen species play a crucial role in the pathogens of diabetes and its complication, this study aims to examine the effects of resveratrol and its derivative on alloxan induced diabetics. Statistical analysis showed a significant decrease in SOD , GSH, Gpx, Zn and Cu concentration and significant increase in MDA level on diabetic rabbits p<0.05 , however diet supplemented with resveratrol derivative induced a significant increase of copper and zinc 1.43±0.01 ppm, 0.79 ± 0.02 ppm respectively after sixth weeks of treatment as compared with control 1.13 ± 0.03ppm, 0.66 ± 0.01ppm respectively. . The administration of resveratrol derivative to rabbits resulted in the significant decrease of MDA levels after three weeks to 2.42±0.08 nanomole /ml and 1.56±0.02 nanomole/ml after sixth weeks as compared with control group 2.43 ± 0.2778 nanomole /ml. The diabetic rabbits presented a significant increase in the SOD activity after treatment by resveratrol derivative after sixth weeks 4.24 ± 0.02 U/ml as compared with control during the experimental period, glbcimide treated group show a significant decrease in SOD activity after sixth weeks . In addition, there was a significant increase in Gpx activity119.77±0.01 U/L in resveratrol derivative treated group after sixth weeks. In infected animals was a decrease level of GSH when drug and purified resveratrol treated rabbits, while resveratrol derivative has normal value after three weeks of treatment 11.17 ± 0.03 μ mole/L and 19.82 ±0.01 μ mole/L after sixth weeks. The results suggest thatresveratrol derivative may be helpful in preventing diabetic complications in rabbits.

 

Keywords: antioxidant enzymes, diabetic disease , oxidative stress , resveratrol.

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[12]. Keaney JFJr, Larson MG, Vasan RS and Wilson PWF (2003).Obesity and systemic oxidative stress :clinical correlates of oxidative stress in the Framingham study . Arterioscler. Thromb, Vasc.Biol.23, 434-439.
[13]. Shah PK and Patel J.A. (2010) Resveratrol and its biological actions .Review article .4(1):15-21.
[14] Harbone JB. (1984). Phytochemical methods, chapman and Hall London.
[15]. Al-Jumaily EF, Al-bayati RA and Shafiq ZA (2012). Antidiabetic and antihyperglycemia effects of Resvertrol and its Derivative on Alloxan diabetic rabbits. IOSR J of Pharmacy Vol.2, 50-58.

 

Paper Type	::	Research Paper
Title	::	Thermal, X-Ray Diffraction, Spectral and Antimicrobial Activity of Bivalent Metal (Zn, Cd, Hg, Pb and Ag) Chelates Of 1, 2 Naphthoquinone – 2, Oxime,
Country	::	India
Authors	::	N. R. Gonewar,||V. B. Jadhav,||S.S. Sakure ||K. D. Jadhav|| R. G. Sarawadekar
Page No.	::	10-17
Paper Index	::	DOI : 10.9790/3013-31201017
ANED	::	DOI : 05.3013/0312010017

Objectives: Five metal chelates of the type M [NQO] 2 where M = Hg, Cd, Zn, Pb: NQO = 1, 2 napthoquinone – 2, oxime and Ag (NQO) have been synthesized. All chelates have been characterized by modern methods such as elemental analysis, FTIR, Electronic spectra. 1H & 13C NMR, Thermogravimetry, Differential scanning calorimetry and electron microscopy with EDAX analysis of chelates were carried out. All chelates are found to be coloured and mercury, lead, zinc and cadmium chelates are octahedral while silver chelate has been assigned square planer. These chelates are thermally stable up to 7000C and all are crystalline in nature. Their particle sizes are in the range of 30-50 nm. The ligand and the metal chelates have been screened for antimicrobial activity on gram positive and gram negative bacteria and fungi and the results are compared with cisplatin as standard.

 

Keywords: 1-2 naphthoquinone -2, oxime, X-ray diffraction, IR, NMR, SEM, Antimicrobial activity, Electronic spectra

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Paper Type	::	Research Paper
Title	::	IIn Vitro CYTOTOXIC STUDY FOR PARTIAL PURE LIGNAN EXTRACT FROM FLAX SEED (Linum usitatissirnurn) ON RHABDOMYOSARRCOMA (RD) AND MURINE MAMMARY ADENOCARCINOMA (MMA) CELL LINES
Country	::	Iraq
Authors	::	Essam F. Al-Jumaily||Auday A. Mahdi
Page No.	::	18-24
Paper Index	::	DOI : 10.9790/3013-31201824
ANED	::	DOI : 05.3013/0312018024

Objectives: The lignan (Secoisolaricinesinal diglucoside, SDG), was obtained from flaxseed ( Linum usitatissirnurn) appear to act as both anti-estrogens and weak estrogens. The anti-estrogenic potential of lignans has been postulated to protect against hormone sensitive cancers such as breast, prostate and colon cancer. The aim of this study was to examine the cytotoxic effect of partial pure lignan on some cell lines including, the Murine Mammary Adenocarcinoma, Rhabdomyosarcoma RD, L20B and normal cell line (Rat Embryo Fibroblast REF). Four concentrations of partial pure SDG which obtained by using alcohol hydrolysis dioxin / ethanol flaxseed extraction and liquid/liquid partition, have been prepared 5, 10, 15, 20 μM with different of exposure periods 24, 48, 72 h. These parameters have been applied on all cancer and normal cell line. The highest percentage of inhibition appears in 5, 10,15 μM at 24 h (60.11, 49.99, 50.28 %) respectively on RD cell line when treated with partial pure. and the with same concentrations this percentage decreases without any significant difference. In case of L20B cell line in 72 h, the inhibitory effect of partial pure at 5,10 μM, 57.67 and 52.11 % respectively. While the cytotoxicity of partial pure SDG on ANM3 cell line appeared in 24h at 15um which was 64.88%. The lignans are growth inhibitors of colon tumor cells and suggest act through mechanisms other than anti-estrogenic activity.

Keywords: Lignan (Secoisolaricinesinal diglucoside), matairesinol, Murine mammary adenocarcinoma, Rhabdomyosarcoma RD, L20B cell lines, Inhibitory effect, growth inhibitors.

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[9]. Thompson, L.U.; Seid, M.M.; Rickard, S.I.; Orcheson, L. and Fong, H.H.S. (1996). Antitumorigenic effect of a rnammalian lignan precursor from flaxseed. Nutr Cancer 26: 159-165.
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Paper Type	::	Research Paper
Title	::	Assessment Of The Bacteriological Quality Of Ice Cream Offered For Public Consumption In Bauchi. Nigeria
Country	::	Malaysia,
Authors	::	Moshood A. Yusuf, ||TengkuHaziyamin Abdul ||Tengku Abdul Hamid||,Mr. Moshood A. Yusuf
Page No.	::	25-30
Paper Index	::	DOI : 10.9790/3013-31202530
ANED	::	DOI : 05.3013/0312025030

30 samples of locally produced and industrially produced ice cream were examined for the presence of bacteria contaminants. Bacteria count was carried out on the different samples using pour plate method. Isolates were identified using standard procedure. Different types of bacteria isolated include Bacillus cereus, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Salmonella typhi. The total bacteria count per ml of the locally produced ice cream sample range from 1.44 – 3.0 4 and the total bacteria count per ml of the industrially produced ice cream sample range between 3.03 - 8.83cfu/ml. The mean count of locally produced and industrially produced ice cream was 2.04 and 5.33 respectively, therefore industrially produced ice cream is more hygienic than locally produced ice cream.The presence of possible pathogenic organisms in the analyzed ice-cream sample should be viewed with concern by the consumers, producing company and the Government since food poisoning by Staphylococcus sp, Bacillus sp and Salmonella sp is possible through consumption of contaminated ice-cream.

 

Keywords:- ice cream, mean count, pathogenic organisms

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Paper Type	::	Research Paper
Title	::	Synthesis And Characterization Of Nano Structured (4- Aminopyridine) Copper (Ii) Acetate Chloride
Country	::	India
Authors	::	A. Sinthiya|| Babu Varghese
Page No.	::	31-36
Paper Index	::	DOI : 10.9790/3013-31203136
ANED	::	DOI : 05.3013/0312031036

The greenish blue [C5H6N2]Cu[C4H6O4]Cl2 particles have been prepared at the room temperature. The grown particles were observed using high-resolution transmission electron microscopy-HRTEM. The results show that, the as-grown particles have the nano tube like structure with breadth 70.9 nm size, and have the structure of 4-aminopyridine copper II acetate chloride. Using Scherrer equation the calculated crystallite size is 0.0325 nm for K=1 and crystallite size is 0.0293 nm for K= 0.9. Using Williamson and Hall theories the calculated ΔK(nm-1) from FWHM is tabulated. From scatter plot we could identify that the more points strongly tend to cluster around a straight line, and it ensures that there is a linear relationship between the two variables (the higher the correlation). Moreover the line around which the points tends to cluster runs from lower left to upper right, so the relationship between the two variable is positive (direct).The elemental analysis conforms that it has 28.01 % of carbon, 2.73 % of hydrogen , 7.01 % of nitrogen, 21.45 % of oxygen, 18.64 % of copper and 19.08 % of chloride. The calculated molecular mass of the as - grown nano tube like structured particle is 346.65 g/mol

 

Keywords:- Fampyridine, acetate, copper chloride, nano tube, HRTEM,Scherrer formula, Williamson and Hall plot

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Paper Type	::	Research Paper
Title	::	Effect Of Pgf2α On Conception Rate In Suboestrus Dairy Buffaloes
Country	::	INDIA
Authors	::	L. Ramsingh|| K.Muralimohan
Page No.	::	37
Paper Index	::	DOI : 10.9790/3013-312037
ANED	::	DOI : 05.3013/0312037037

Suboestrus is the major causes of infertility in post partum buffaloes which leads to economic losses due to increased service period. The present study was undertaken to evaluate efficacy of PGF2αin suboestrus dairy buffaloes.

 

Keywords: PGF2α, Dairy buffaloes, Suboestrus

[1]. Gautam, A.P., Kharche, K.G. and Thakur, M.S. (1990)…. Indian J. Anim. Repord. 11:164
[2]. Pant, H.C. and Singh, B.P. (1991)…. ibid., 12:55
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Paper Type	::	Research Paper
Title	::	Mukkuddu Maathrai (Triple Tablet), An Indigenous Medicine Elevates Serum Prolactin Level Of Female Rats (Rattus norvegicus)
Country	::	Sri Lanka
Authors	::	Kanisra Mary F|| Eswaramohan T||Surendran S N||Murugananthan A||Romakeswaran P
Page No.	::	38-42
Paper Index	::	DOI : 10.9790/3013-31203842
ANED	::	DOI : 05.3013/031203804

The Mukkuddu Maathrai is a mixture of Kasturi, Saffron and Korosanai. Ayurvedic doctors in Jaffna peninsula have been prescribing this mixture to patients for their good health. The prolactin (PRL) can be used as a marker in hypothalamo-pituitary axis. The objective of the present study was to determine the effect of Mukkuddu Maathrai treatment on serum prolactin level of female rats at estrus stage. Female rats were maintained under uniform conditions. Stages of oestrus cycle were determined by using vaginal smear cytology. Initial blood sample was collected by tail bleeding at the estrus stage and were treated with oral feeding. Tests and controls were fed with 5% w/v of the Mukkuddu Maathrai mixture solution and distilled water alone respectively. Treatments were done continuously as once per day until one oestrus cycle complete. At the next estrus stage, second blood sampling was performed. Serum PRL levels were measured by enzyme immunoassay and the results were compared by using paired't' test at 95% confident limits. All experiments were conducted with their duplicates. During the treatment, prolactin secretion increased significantly in female rats at estrus stage. Mukkuddu Maathrai treatment triggers hypothalamo-pituitary axis of female rats and thus influences on serum prolactin level.

 

Keywords:- Estrus, Kasturi, Korosanai, Prolactin, Saffron..

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Paper Type	::	Research Paper
Title	::	In Vitro studies on changes in selected biochemical parameters and morphology of Sertoli cells in mice overexpressing bovine growth hormone
Country	::	India
Authors	::	M. Bhaskar
Page No.	::	43-48
Paper Index	::	DOI : 10.9790/3013-31204348
ANED	::	DOI : 05.3013/0312043048

The aim of the present study was to investigate the changes in selected biochemical variables in Sertoli cells isolated from testis of mice (PEPCK-bGH) overexpressing growth hormone. Significant increase in the levels of total proteins (108.33%; p<0.001), lactic acid (95.23%; p<0.001), DNA (155.42%; p<0.001) and RNA (90.00%; p<0.001) were observed in transgenic Sertoli cells as compared to normal Sertoli cells. Further, cultured transgenic Sertoli cells exhibited distinct nucleus over a period of 5 days and on day 7, exhibited accelerated proliferation as compared to normal Sertoli cells. From the results, it can be concluded that growth hormone at least in part mediates modulation of selected biochemical variables to influence Sertoli cell morphology thereby spermatogenesis in mice.

 

Keywords: DNA, lactic acid, PEPCK-bGH transgenic mice, RNA, Sertoli cells, total proteins

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Paper Type	::	Research Paper
Title	::	Effect of Silver Nanoparticles On Energy Metabolism In Selected Tissues Of Aeromonas Hydrophila Infected Indian Major Carp, Catla Catla
Country	::	India
Authors	::	T. Kiran Reddy,||S. Janardana Reddy ||T.N.V.K.V. Prasad
Page No.	::	49-55
Paper Index	::	DOI : 10.9790/3013-31204955
ANED	::	DOI : 05.3013/0312049055

The aim of the present study was to evaluate the effect of silver nanoparticles (AgNPs) on energy metabolic enzymes in selected tissues of Aeromonas hydrophila injected Indian major carp, Catla catla. Fishes exposed to 1/10th concentration of AgNPs (100 μg/l) did not alter lipid peroxidation levels as compared to 1/5th concentration of AgNPs in gills, liver and kidney of fishes. Therefore, 1/10th concentration of AgNPs was selected for subsequent experiments. Significant decrease in the activity levels of succinate dehydrogenase with a significant increase in the activity levels of glucose-6-phosphate dehydrogenase and lactate dehydrogenase in gills, liver and kidney of A. hydrophila infested fishes as compared to controls over a 14-day exposure period. On the other hand, exposure of bacterial infected fishes to 1/10th concentration of AgNPs reversed the activity levels of enzymes in selected tissues as compared to bacterial infected fishes. However, no significant changes were observed in the activity levels of selected enzymes in tissues of fishes exposed to 1/10th concentration of AgNPs alone over controls. From the results, it can be concluded that A. hydrophila injection leads to prevalence of anaerobic conditions to meet the demand of energy in tissues whereas AgNPs through their antibacterial properties mitigate A. hydrophila induced changes in oxidative metabolism thereby energy crisis in tissues of fishes.

 

Keywords:- A. hydrophila, Catla catla, lipid peroxidation, oxidative enzymes, silver nanoparticles

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