Obesity, one of the greatest public health challenges of 21st century, is related to several chronic conditions such as type II diabetes, hypertension, dyslipaedaemia and cardiovascular diseases and renal pathologies. Individuals with increased abdominal obesity are found to have an atherogenic triad, associated with adverse cardiovascular outcomes. Human obesity is characterized by marked sympathetic activation. Adiposity is associated with chronic low-grade systemic as well as inflammation of the hypothalamus. In women, early onset of obesity favors the development infertility in the adult age. In men, obesity is associated with low testosterone levels, reduced spermatogenesis and erectile dysfunction. Lifestyle modifications, drugs & surgical intervention may prove to be fruitful in treatment of obesity.

 

Keywords: Obesity, health hazards.

[1.] Elizabeth A Valente, Megan E Sheehy, Joshua J Avila, Julie A Gutierres, Matthew J Delmonico, Ingrid E Lofgren. The effect of the addition of resistance training to a dietary education intervention on apolipoproteins and diet quality in overweight and obese older adults Clinical Interventions in Aging 2011:6.235-241.
[2.] Yadav K, Krishnan A. "Changing patterns of diet, physical activity and obesity among urban, rural and slum populations in north India". Obes Rev 2008; 9 (5): 400–8. doi:10.1111/j.1467-789X.2008.00505.x. PMID 18627500.
[3.] Guyton AC, Hall JE. Dietary balances; Regulation of feeding; Obesity and Starvation; Vitamins and Minerals. Textbook of Medical Physiology. 11th Ed. 2007; Elsevier, Saunders Inc, New Delhi, India: 843-857.
[4.] Mahbubur R, Abbey B. Accuracy of Current Body Mass Index Obesity Classification for White, Black, and Hispanic Reproductive-Age Women. Obstetrics & Gynecology 2010; 115 (5):982-988.
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A simple, rapid, sensitive and specific Ultra performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous estimation and validation of tablet dosage form containing glimepride (GLIM), metformine (MET) and pioglitazone (PIO) using Tolzamide (TOLAZ) as an internal standard (IS). The chromatography separation was achieved with Waters ACQUITY HSS C18, 1.8 μm, 2.1X50 mm column with mobile phase containing acetonitrile (A) & 1% ammonium acetate buffer (B) (pH 2.5 adjusted with trifluoro acetic acid) with gradient mode Gradient mode [2 min: 20 A : 80% B, 2-4 min: 70% A : 30% B, 4-5 min,80% A : 20% B, 8-10 min 90% A: 10% B]. The flow rate was 0.4 mL min-1 column maintained at 250C and the injection volume was 2 μl. The selected chromatographic condition were found to effectively separate glimepride, metformine and pioglitazone with retention time of 3.17, 0.425, 2.3 min, respectively. The proposed method was found to be rectilinear over the range of 2-12 ng mL-1, 500-3000 ng mL-1 and 15-90 ng mL-1 for glimepride, metformine, and pioglitazone respectively. The signal intensities obtained in both positive and negative ion mode all drugs including internal standard found to be much higher in positive ion mode (M +H)+ parent ions at m/z, 491.11, m/z 129.87, m/z 357.04 and m/z 312.04 respectively in QUATTROZQ full scan mass spectra. The present method was validated as per ICH guidelines with respect to precision, specificity, linearity, limit of detection, limit of quantification, accuracy, and robustness and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined pharmaceutical dosage forms.

Keywords: UPLCMS, glimepride, metformine, pioglitazone and tolazamide simultaneous estmation

[1.] Cheng, J.T., Huang, C.C., Liu, I.M., et al., Novel mechanism for plasma glucose lowering action of metformin in streptozotocin-induced diabetic rats Diabetes, 2006: 55; 819-825.
[2.] Lebovitz H.E., Oral antidiabetic agents, Medical Clinical North America, 2004: 88; 847-863.
[3.] David Bell S.H., Type 2 diabetes mellitus: what is the optimal treatment regimen, The American Journal of Medicine, 2004: 116(5); 23-29.
[4.] Pandit V., Gorantla R., Devi K., et al. Preparation and characterization of pioglitazone cyclodextrin inclusion complexes. J. Young Pharm. 2011: 3; 267–274.

More than 1.2 billion Muslim practice fasting from dawn to sun set through the whole month of Ramadan. Cancer patients who are on active chemotherapy therapy treatment are usually feeling impressed whether or not to keep fasting with no data to support the possible hazard or potential benefit, Here we report data about representative samples from our patients who had been followed during the fasting time and use them as referral control for themselves while receiving the chemotherapy without fasting. Subjective symptoms and objective laboratory results are reported. To our knowledge this is the first ever study in the field

[1] WCRF/AICR; Food, nutrition and the prevention of cancer: a global perspective:. World Cancer Research Fund/ American Institute for Cancer Research 1997.
[2] Dirx M. , Zeegers M. , Dagnelie P. , van den Bogaard T. and van den Brandt P. " energy restriction and the risk of spontaneous mammary tumors in mice: a meta-analysis" Int. J. Cancer 2003; 106, 766–770
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[4] Hursting, S. D., Perkins, S. N., and Phang, J. M. "Caloric restriction delays spontaneous tumorigenesis in p53 knockout transgenic mice." Proc. Natl. Acad. Sci. USA, 1994; 91: 7036-7040.
[5] Ross, M. H. & Bras, G. "Lasting influence of early caloric restriction on prevalence of neoplasms in the rat." J. Natl. Cancer Inst. 1971; 47: 1095–1113
[6] Weindruch, R. & Walford, R. L. "Dietary restriction in mice beginning at 1 year of age: effect on life span and spontaneous cancer incidence." Science 1982; 215: 1415–1418.
[7] Raffaghello L., Lee C., Safdie F.M., Wei M., Madia F., Bianchi G., and Longo V.D. "Starvation-dependent differential stress resistance protects normal but not cancer cells against high-dose chemotherapy". Proc. Natl. Acad. Sci. USA, 2008; 105: 8215-8220

Novel Tetrazole imine and its binary Cu(II) and Ni(II) complexes were synthesized and characterized by employing spectro - analytical techniques viz; Mass, UV, IR, 1H NMR, elemental analyses, thermo-analytical methods, SEM and magnetic susceptibility measurements. The equilibrium studies were carried out using pH-metric technique to get an insight of the number of dissociable protons and protonation sites in candidate compound. The pH-Metric studies were also carried out in presence of metal ions to establish the formation of corresponding metal complexes in solution. To understand the frontier orbitals in title imine compound suitable for metal ion coordination, the computational studies were carried out by employing HyperChem 7.5 tools. The energies of HOMO and LUMO frontier orbitals were computed with geometry optimized molecules. The antimicrobial studies carried out by screening the tetrazole imine base and their metal complexes against bacteria inferred positive results. The DNA cleavage studies on title compound and its metal complexes using PBR322 DNA indicated that the tetrazole imine base has no role in cleavage of DNA and in its metal complexes, only Cu (II) complex played marked role in cleavage

 

KEY WORDS: Novel tetrazole imines Binary Cu (II) and Ni (II) complexes, Computational studies, DNA cleavage, Spectro-analytical studies

[1] Wittenberger, S. Journal of 0rganic Preparations and Procedures International 26, 1994, 499.
[2] Ford, R. E.; Knowles, P.; Lunt, E.; Marshall, S. M.; Penrose, A. J.; Ramsden, C. A.; a. Summers, A. J. H.; Walker, J. L.; Wright, D. European Journal of Medicinal Chemistry ,29, 1986, 538.
[3] Peet, N. P.; Baugh, L. E.; Sundler, S.; Lewis, J. E.; Matthews, E. H.; Olberding, E. L.; a. Shah, D. N. Journal of Medicinal Chemistry ,29, 1986, 2403.
[4] Girijavallabhan, V.M.; Pinto, P.A.; Genguly, A.K.; Versace, R.W. European Patent EP a . 274,867, 1988; Chemical Abstract, 1989,110 23890.
[5] (a) Akimoto, H.; Ootsu, K.; Itoh, F. European Patent EP 530,537, 1993; Chemical abstract a. 119, 1993, 226417. (b) Taveras, A.G.; Mallams, A.K.; Afonso, A. International Patent WO b. 9,811,093, 1998; Chemical abstract 128, 1998, 230253.

Inflammation is body's attempt at self protection characterized by five cardinal signs; it may be acute or chronic. NSAID(non steroidal anti-inflammatory drugs are the commonly used to reduce the inflammation. traditional Indian system of medicine mentions neem(Azadirachta Indica)to have many medicinal properties. so the present study was carried out to access the anti-inflammatory effect of neem.Materials and methods: Albino rats were used; they were divided into three groups. Control group treated with normal saline, standard treated with Indomethacin and test drug used was neem oil. For acute inflammation; Carregenan induced rat paw edema inhibition method and for sub acute inflammation: cotton pellet granulation method. Ulcer index of Indomethacin and test compound were also studied.Results: it is found that neem oil showed significant anti-inflammatory effect in both acute as well as chronic inflammation, it was also found to have low ulcerogenic potential compared to Indomethacin, hence can be safely used as a potent anti-inflammatory agent

 

KEYWORDS: Neem (Azadirachta-Indica), Inflammation, Indomethacin, Carregennan, Cotton pellet

[1] Cotton RS ,Kumar V and Robbins Sl. Robbins pathologic basis of disease.5th edn Philadelphia B Sauderers company ,1994.p.53-4
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[3] Dey NC, Debashish S, Pey PK edn. Textbook of pathology; New central book agency ltd,19995:p.6.1-6.59
[4] Rubin E. Essential pathology, 2nd edn, Chapter-2 inflammation, Philadelphia: Lippincort Williams & Wilkins, 2001: p-24-46.
[5] Satoskar RS, Bhandarkar SD, Ainapur SS, Angiotensin, Kinins, Leukotrienes prostaglandins and cutokiones, 18th edn. Bombay India; Popular Prakashan pvt. Ltd. 2003 p 333-9
[6] Tripathi KD. Essentials of medical pharmacology, 5th edn .prostaglandin Leukotrienes, jaypee pub Pvt Ltd, 2004 P 156-166

Oral diseases are major health problems among which dental caries and periodontal diseases are the most important preventable diseases. When these conditions are not arrested,they cause formation of periodontal pockets.Periodontal diseases havebeen linked to bacteria like Actinobacillus actinomycetemcomitans, Treponema denticola, T.socranskii, P.intermedia and Porphyromonas gingivalis.[1] The need to develop an alternative prevention and treatment option to antibacterial agents, is to avoid side-effects of the antibiotics and the increased resistance of the bacteria. Hence, natural herbs and ayurvedic drugs have become a subject of importance.[2]

 

KEYWORDS: Aloe-vera,bacteria,herbs,periodontal disease

[1] 2 and 3) Jai Ganesh and Lakshmi (2011). Unfolding gift of nature- Herbs for the management of Periodontal disease. Journal of Pharmacology research. 1:2576
[2] Grindwit Sastrvaha, Pusadee Yotnuengnit, Prakongsiri Booncong and preecha Sangtherapitikul. Adjunctive Periodontal treatment with Centella asiatica and Punica granatum extracts. 2:107
[3] Sharma P. C., Yelne M. B., Denni T. J., Database on Medicinal Plants used in Ayurveda, Volume 1, Central Council For Research in Ayurveda and Siddha, Dept.of ISM & H, Min. of Health & Family Welfare, Govt. of India, 216-224. 2000
[4] Jai Ganesh and Lakshmi(2011) . The unfolding gift of nature- Herbs for the management of periodontal disease. Journal of Pharmacology research. 3:2577

The present paper reviews trends in the development and approval of new drugs. The pharmaceutical industry continues to face the problems of high costs of new drug development, FDA's regulatory regime, and competition from generic drugs. At the same time, consumers' expectations regarding drug efficacy and safety have continued to increase. This explains why it has become increasingly harder for drug firms to invent new molecules during the last three decades. A few of the resolutions to these problems include strategic drug development data generation, lifecycle planning for a new molecular entity and mergers and acquisitions involving biotechnology and pharmaceutical companies. Moreover, cooperation amongst FDA's review division and industry is not at desired level to seek development of new safety and efficacy models for enhance productivity in the industry.

 

KEYWORDS: Drug Development, FDA, New Molecular Entities, Regulatory Strategies, Trends in New Drug Development

[1] DiMasi et al., J. (1995). Research and Development Costs for New Drugs by Therapeutic Category: A Study of the U.S. Pharmaceutical Industry. PharmacoEconomics, 152-169.
[2] DiMasi, J. (2001). New drug development in the United States from 1963 to 1999. Clinical Pharmacology & Therapeutics, 69(5), 286–296.
[3] DiMasi-4, J., & Grabowski, H. (1995). R&D Costs, Innovative Output, and Firm Size in the Pharmaceutical Industry. International Journal of the Economics of Business, 201-221.
[4] Grabowski-4, H. G., Vernon, J. M., & DiMasi, J. (2002). Returns to R&D on 1990s new drug introductions. PharmacoEconomics, 20 supplement 3: 1-29 (19).

Rubber dust particles affect the respiratory system bydirectly enteringinto the respiratory tract while breathing. We analysed the effect and the impact of duration of exposure to these dust particles on the lung volumes by pulmonary function test (PFT) in 4 groups of 60 male employees in a rubber factory. Group 1 - Controls.Group 2, 3 and 4 - test groups with1-3years, 4-7 years and 8-11 years exposure to rubber dust particles. Forced vital capacity (FVC), forced expiratory volume in one second, (FEV1), FEV as a percentage of FVC (FEV1/FVC), forced expiratory flow 25-75% (FEF 25-75%), peak expiratory flow rate (PEFR) and maximum voluntary ventilation (MVV) were assessed. Themean values of these variables(except for FVC) showed significantdecline in Group 2, 3 and 4 (p < 0.000) compared to that of Group 1 and the decline was the maximum in Group 4 showing that lung function is affectedby the rubber dust particles and the severity of the effect is duration-dependent,less in short time-exposure and more in longtime-exposure. Knowing this fact may create awareness among the employers and the employees of rubber factories to plan for preventive measures.

 

KEY WORDS: Duration dependent, Lung volumes, Pulmonary function test, Rubber factory, Spirometry

[1] "Pollution - Definition from the Merriam-Webster Online Dictionary". Merriam-webster.com. http://www.merriam-webster.com/dictionary/pollution.

[2] Levy D, Sheppard L, Checkoway H, Kaufman J, Lumley T, Koenig J, Siscovick D, Levy D. 2001 . A case-crossover analysis of particulate matter air pollution and out of hospital primary cardiac arrest, Epidemiology (Cambridge, Mass) 2(12): 193-9.

[3] Ohya, N.; Tanaka, Y.; Wititsuwannakul; Koyama, T.Activity of rubber transferase and rubber particle size in Hevea latex.Journal of Rubber Research 2000 Vol. 3 (4): 214-221

[4] Sullivan, J.B., Jr., M. Van Ert, and R. Lewis. 1992. Chemical hazards in the tire and rubber manufacturing industry. In J.B. Sullivan, Jr. and B.R. Krieger (eds.). Hazardous materials toxicology, clinical principals of environmental health. Williams and Wilkins, Baltimore, MD. Pp. 516-532.

[5] Varigos, G.A. and D.R. Dunt. 1981. Occupational dermatitis. An epidemiological study in the rubber and cement industries. Contact Dermatitis 7: 105-110.

To objectify smoking and its connection with various diseases and conditions are used analytical markers. The aim of our study was to estimate smoking and its intensity by determination of thiocyanates in blood serum and / or urine at high school students, university students and children with essential hypertension / PAH /. In the group of children with high blood pressure (BP) were also determined lipid parameters. Thiocyanates are determined in the urine of 86 students aged 15-18 years, and 10-15 year olds 41 - hypertensive. 84 medical students - 18-22 years old were studied serum and urine thiocyanate. We found that 44.49% of students and 50 % of students are smokers , and 47.4% of smokers and 50 %, respectively smoke more than 10 cigarettes a day .The differences in thiocyanates levels in serum and urine in the groups of non - smokers , smoking 10 cigarettes a day and smoking more than 10 cigarettes a day students are statistically significant. Children with hypertension smokers are 31.02%. They have statistically higher levels of thiocyanates in urine, higher values of cholesterol and triglycerides and lower HDL-cholesterol, compared to non - smokers with hypertension.The results supported by published data shows the relationship between smoking, high BP and lipid profile in infancy. Given that smoking not only affects the cardiovascular system and overall development of the child's body , it is necessary to introduce health programs and strategies for family life improvement, for prevention of smoking at school age and to provide support to those who want to quit smoking.

 

KEY WORDS:, smoking, thiocyanate, hypertension, children.

[1] Jarvis MJ, Sims M, Gilmore A, Mindell J. (2012). Impact of smoke-free legislation on children's exposure to secondhand smoke: cotinine data from the Health Survey for England. Tob Control, 21(1):18-23
[2] . Buratti M, Xaiz D, Caravelliand G, Colombi A. (1997). Validation of urinary thiocyanate as a biomarker of tobacco smoking. Biomarkers, 2 (2):81-85
[3] Hongbing Wang, Michikazu Sekine, Hiroshi Yokokawa, Shimako Hamanishi, Michio Sayama, Yuchi Naruse, Hideaki Nakagawa, Sadanobu Kagamimori. (2012). Serum thiocyanate concentration as an indicator of smoking in relation to deaths from cancer. Environmental Health and Preventive Medicine 6(2):88-91
[4] Dunbar A, Gotsis W, Frishman W. (2013) Second-hand tobacco smoke and cardiovascular disease risk: an epidemiological review.Cardiol Rev. 21(2):94-100

Decalepis hamiltonii is a climbing shrub with aromatic tuberous roots distributed in Southern parts of Peninsular India. Its tuberous roots are widely used as a health drink and are well known for its medicinal properties. D. hamiltonii is one of the important plants in Ayurvedic system of medicine in India and are used in curing various diseases like stomach disorders, gastric ulcers and to stimulate appetite. It is used as a food and health drinks, phytochemistry, pharmacology and conservation is required. The tubers have reported antimicrobial, antipyretic, antiulcer, antidiabetic, antioxidant, anti-inflammatory, chemoprotective, cytoprotective, insecticidal, neuroprotective and hepatoprotective activities. Natural seed germination is very low in this species, that is, 6% because of hard seed coat, less seed dormancy period and due to self-incompatibility. In vivo and in vitro conservation methods have been standardized to this endangered plant by developing rapid micro propagation techniques. Among different plant growth regulators tested, 6-benzylaminopurine (BAP) played a significant role in shoot multiplication, whereas, indole-3- butyric acid (IBA) and silver nitrate (AgNO3) influence rooting efficiently. In the present study, Antioxidant activity, total phenol and flavonoids estimation of this Tuber part of Decalepis hamiltonii was evaluated for free radical scavenging activity using 2,2-diphenylpicryl-1-picrylhydrazyl (DPPH),The FT-IR spectra results shows that quercitin is present in this plant. Hence this tissue culture plant has rich source of antioxidant properties. If this plant started growing by micro propagation, it could be commercially viable as a nutritive and therapeutic source.

 

KEYWORDS - Decalepis hamiltonii, Micro propagation, total flavanoid content (TFC), Total Polyphenol content, Antioxidant activity, Quercitin, FTIR

[1] Thornell K, Vedavathy S, Mulhooll DA and Crouch NR, Parallel usage pattern of African and Indian periplocoids corroborate phenolic root chemistry, South African Ethnobotany, , 2(2), 2000,17-22.
[2] Nayar RC, Shetty JKP, Mary Z, Yoganarasimhan SN. Pharmacognostical studies on the root of Decalepis hamiltonii Wight & Arn. and comparison with Hemidesmus indicus (L.). Proc. Indian Acad. Sci. 87B, 1978, 37-48.
[3] Shefali K, Vandana P, Akhilesh S, Sandhya S. Pharmacognosy, phytochemistry and pharmacology of Decalepis hamiltonii Wight & Arn. In: Dwivedi (Ed) Recent advances in prospects and potential of medicinal plants. 2009, pp. 156-161
[4] Vedavathy, S. Decalepis hamiltonii Wight & Arn. – An endangered source of indigenous health drink. Natural Product Radiance, 3 (1), 2004, 22-23.
[5] Ebadi, M. Pharmacodynamic basis of Herbal Medicines (CRC Press, Boca Raton. 2002), pp: 86.

INTRODUCTION : Hepatitis C Virus (HCV) is the leading cause of death throughout the world. The standard of care for the treatment of chronic hepatitis C is combination therapy with Pegylated Interferon (PEG-IFNα 2a) and Ribavirin (RBV). There currently exists no systematic explanation for these genotype-specific differences in clinical outcome. Furthermore, whether factors that govern outcome for one genotype play a similar role in other genotype remains to be fully explored. Hence, the present study was taken in consideration of the factors emphasizing their impact on the sustained virological response (SVR) against HCV genotypes.

 

METHODOLOGY : A total of 50 patients (Age, Mean: ±SD 42.53 ± 12.6) having chronic hepatitis C genotype 3 and genotype 1 who showed positive result for HCV-RNA for more than 6 months were treated with combination therapy of PEG-IFNα 2a and RBV. All the patients were followed up for 48 weeks of post treatment and varied virological response was recorded in respect to the HCV genotypes, subtypes and biological parameters.

 

RESULTS : In present study, we have observed that males had a better SVR and EVR as compared to females in both the genotypes (genotype 1 and genotype 3) and among the non responders there were less males as compared to females. It was also seen that there were less females who showed EVR and SVR as compared to males.

 

CONCLUSION : Our study has demonstrated that EVR, RVR, NR and most importantly SVR are important factors for the achievement of complete virological response against HCV genotypes and subtypes.

 

KEYWORDS : Virological response, HCV, Pegylated Interferon-α and Ribavirin

[1] National Institutes of Health Consensus Development Conference Statement: Management of hepatitis C 2002. Gastroenterology 123: (June 10–12, 2002), 2082–2099.
[2] Verna EC, Brown RS, Hepatitis C virus and liver transplantation. Clin Liver Dis 10, 2006, 919–940.
[3] Hofmann WP, Herrmann E, Sarrazin C, Zeuzem S, Ribavirin mode of action in chronic hepatitis C: From clinical use back to molecular mechanisms. Liver Int 28, 2008, 1332–1343.
[4] Feld JJ, Hoofnagle JH, Mechanism of action of interferon and ribavirin in treatment of hepatitis C. Nature, 436, 2005, 967–972.
[5] Mangia A, Santoro R, Minerva N, Ricci GL, Carretta V, et al., Peginterferon alfa-2b and ribavirin for 12 vs. 24 weeks in HCV genotype 2 or 3. N Engl J Med 352, 2005, 2609–2617.
[6] Shiffman ML, Suter F, Bacon BR, Nelson D, Harley H, et al., Peginterferon alfa-2a and ribavirin for 16 or 24 weeks in HCV genotype 2 or 3. N Engl J Med 357, 2007, 124–134.

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefotaxime sodium in bulk and pharmaceutical formulation. The solvent used throughout the experiment was methanol and water. Absorption maximum (λmax) of the drug was found to be 260 nm. The quantitative determination of the drug was carried out at 260 nm and Beer's law was obeyed in the range of 10-30μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.025x + 0.0028 with correlation coefficient R2of 0.9995. The recovery values for cefotaxime sodium ranged from 99.95% - 100.21%.The percent relative standard deviation (RSD %) of interday precision range was 0.099 – 0.140% and intraday precision range was 0.098 – 0.132%. The limit of detection and limit of quantification was 0.079μg/mL and 0.154μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.142 – 0.221%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.

 

KEYWORDS: UV-Vis Spectrophotometer, Method Validation, Recovery studies

[1] Williams JD, Naber KG, Bryskier A, Hoiby N, Gould IM, Periti P, Giamarellou H, Rouveix B. Int. J. Antimicrob. Agents. 2001;17:443.
[2] Martindale, The extra pharmacopeia, The pharmaceutical Press,London Edition.29,1989,151.
[3] O.Layne and M.D,Gentry, Cefotaxime and prophylaxic; New approaches with a proven agent.Am.J.Med.,88,1990,S32.
[4] Nanda RK, Bhagwat VV, Potawale SE, Hamane SC, Development and validation of a HPTLC method for simultaneous densitometric analysis of Cefotaxime Sodium and sulbactam sodium as the bulk drugs and in the pharmaceutical dosage form, Journal of Pharmacy Research, 3(7),
[5] Agbaba D., Zivanov-Stakic. Dand Vladimirov. S., HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms., J .Pharm. Biomed Anal., 1998, 18, 893-898. 2010, 1667-1669.
[6] Iqbal MS, Bahari MB, DarwisY, Iqbal MZ, Hayat A, Gantala V. An RP-HPLC-UV Method with SPE for Cefotaxime in All-in-One Total Parenteral Nutritional Admixtures:Application to Stability Studies. Journal of AOAC International. 2013;96 (2):290-294.