There is much interest in plant based medicine with antioxidant and anti-glycation properties. Formation of advanced glycation end products (AGEs) is a major cause of serious chronic diabetic complications. The objective of this study was to analyze the radical scavenging activity of different solvent extracts and the protein glycation inhibitory potential on fructose mediated non enzymatic glycation by the aqueous leaf extract of Artocarpusheterophyllus(ALEAH). The ALEAH (ripe and raw) was prepared separately according to the preparation of a "Kasaya" in Ayurvedic medicine. The freeze dried powder was used for the investigations. The leaves were soaked in methanol or ethanol and shaken, filtered evaporated in order to obtain the respective extracts..............

Key Words: Antiglycation activity, Artocarpusheterophyllus, radical scavenging activity

[1]. P. PRD, "In Vitro Antiglycation Activity of Some Medicinal Plants Used in Diabetes Mellitus," Med. Aromat. Plants,2(6), 2013, 5–7.
[2]. H. K. I. Perera, J. Poongunran, W. K. V. Kalpani Premadasa, and J. A. V. P. Jayasinghe, "In vitro antiglycation and hypoglycaemic effects of Syzygium cumini leaf extracts," J. Natl. Sci. Found. Sri Lanka, 46( 3), 2018, 281.
[3]. V. P. Singh, A. Bali, N. Singh, and A. S. Jaggi, "Singh, Bali, Singh, Jaggi - Korean J Phyiol Pharmacol - 2014,"18, 2014, 1–14.
[4]. A. Meeprom, W. Sompong, C. B. Chan, and S. Adisakwattana, "Isoferulic acid, a new anti-glycation agent, inhibits fructoseand glucose-mediated protein glycation in vitro," Molecules, 18, 6, 2013, 6439–6454.
[5]. P. Perera, "In vitro study on antiglycation activity, antioxidant activity and phenolic content of Osbeckia octandra L. leaf decoction," … Pharmacogn. …,2(4) ,2013, 198–201.

Here, we studied various preserving media compositions for long-term storage of L. Pasteur rabies virus vaccine strain at –20 and –80ºС in rabies products pharmaceutical manufacturing. Differences in strain infectious activity were established after storage at –80 and –20ºС. Infectious activity of the virus stored at –80ºС was higher than in the samples stored at –20ºС, the differences were statistically significant. During storage at these temperatures within a year (observation period), the highest safety of L. Pasteur rabies virus strain was provided with preserving media based on growth medium without additives and supplemented with sucrose, gelatin, glycerol and DMSO. Considering regulatory requirements and rabies products manufacturing conditions, protective media based on DMEM with 0.1% BSA, both without cryoprotectants and with the addition of 2.5-10% sucrose and storage at –80ºС were recommended for the long-term preservation of the studied rabies virus strain.

Key Words: cell culture, long-term storage, protective media, rabies virus, virus infectious activity, virus safety.

[1]. V.V. Burkova, I.P. Vysekantsev, A.A. Lavrik, Preservation of infectious activity of rabies virus industrial strains stored at various temperatures, Zhivye biokosnye sist, (9), 2014, 1–11.
[2]. F.X. Meslin, M.M. Kaplan, H. Koprowski, editors, Laboratory techniques in rabies. 4th ed. (Geneva: WHO; 1996. 446 p.)
[3]. WHO Expert Committee on Biological Standardization. Recommendations for inactivated rabies vaccine for human use produced in cell substrates and embryonated eggs. In: WHO Technical Report Series – 941. Fifty-sixth report. (Geneva: WHO, 2007) 83–132.
[4]. C.K. Gupta, J. Leszczynski, Gupta RK, Siber GR. Stabilization of respiratory syncytial virus (RSV) against thermal inactivation and freeze-thaw cycles for development and control of RSV vaccines and immune globulin, Vaccine 18(15), 1996, 1417–1420.
[5]. D. Lalošević, V. Lalošević, L.J. Lazarević-Ivanc, I. Knežević, BHK-21 cell culture rabies vaccine: immunogenicity of a candidate vaccine for humans, Dev. Biol. 131, 2008, 421–429.

Aim of the study: The study was designed to monitor the usage of Intravenous (IV), Intramuscular (IM), Intrathecal and Nebulized preparations of restricted antibiotics and the existing resistance pattern in a tertiary care hospital. It is important to know the resistance pattern as resistance among both gram positive and gram-negative organisms become a concern in treating the disease in critically ill patients. Materials and methods: A prospective interventional study was carried out in a 850 bedded tertiary care private corporate hospital over a period of six months (December 2017 – June 2018). All patients receiving IV, IM, Intrathecal and Nebulized restricted antibiotics in inpatient wards were included in this study. The cases were analyzed for appropriateness of the antibiotics chosen based on the dose, frequency, choice of drug for the specific site of infection..........

Key Words: Antibiogram, Bacterial resistance, Clinical intervention, Restricted antibiotic, Utilization pattern.

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INTRODUCTIONUrolithiasis is presence of one or more calculi in urinary system. It is a disease with high recurrence both in human and small animal. Calcium oxalate is a major component of urolithiasis. The aim of this study is to evaluate the activity of combination extract Persea americana Mill and Orthosiphon aristatus (Blume)Miq. as inhibitor crystal calsium oxalate in urine through dissolving mechanism. METHODSThe varian combination was compared with commercial brand (group K+) using artificial urine as solvent. This experiment used 11 group with varian combination Persea americanaMill. and Orthosiphon aristatus (Blume)Miqextract. The measurement of calcium from the solution was used spectrophotometric atomic absorption (AAS).RESULTS AND DISCUSSIONThe result showed that combination of extract Persea americana Mill. and Orthosiphon aristatus was effectively as inhibitor calcium oxalate and the combination of the extract at rasio (1:4) (P8) and (4:1) (P9) had the best potency as the inhibitor urolithiasis.

Key Words: Persea americana Mill, Orthosiphon aristatus(Blume)Miq., kidney stone, calcium oxalate,urolithiasis.

[1]. Abdelwahab SI, Mohan S, Elhassan MM, Al-Mekhlafi N, Adam Mariod A, Abdul AB, et al. 2011. Antiapoptotic and antioxidant properties of Orthosiphon stamineus Benth : Intervention in the Bcl-2-mediated apoptotic pathway. Evidence-based Complementary and Alternative Medicine. Article ID 156765 :1-11. doi:10.1155/2011/156765
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[3]. Adyana IK, Setiawan F, Insanu M. 2013. From etnopharmacology to clinical study of Orthosiphon Stamineus Benth. Int J Pharm Pharm Sci. vol 5, issue 3, 66–73.
[4]. Beghalia M, Ghalem S, Allali H, Belouatek A, Marouf A. 2014. Effect of herbal extracts of Tetraclinis articulata and Chamaerops humilis on calcium oxalate crystals in vitro. Gomal J Med Sci. 2007 ; 5:55-8.

Background: Cost of any drugs can not only judge by simple their price but at the same time efficacy, safety and quality of drug itself determined the actual cost of a drug. Aims and Objectives: Aim of the present study is to compare the cost-effectiveness and safety of Nebivolol and Metoprolol in order to reduce blood pressure. Materials and Methods: The present study includes a total of 90 randomly selected patients with the age ranges from 25 to >65 years (mean= ±12). The drug Metoprolol and Nebivolol is given to the 56 and 34 randomly selected patients respectively to observe the effect and tolerability of drug in respect to their costs. Cost effectiveness was calculated and compared by 1mm fall of Hg by both drugs. Safety and effectiveness was observed clinically..........

Key Words: Hypertension, Pharmacoeconomic, Efficacy, Cost-effectiveness.

[1]. Naish, Jeannette; Court, Denise Syndercombe (2014). Medical sciences (2 ed.). p. 562. ISBN 9780702052491

[2]. Lackland, DT; Weber, MA (May 2015). "Global burden of cardiovascular disease and stroke: hypertension at the core". The Canadian journal of cardiology. 31 (5): 569–71. doi:10.1016/j.cjca.2015.01.009. PMID 25795106.

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[5]. Lau, DH; Nattel, S; Kalman, JM; Sanders, P (August 2017). "Modifiable Risk Factors and Atrial Fibrillation". Circulation (Review). 136 (6): 583–96. doi:10.1161/CIRCULATIONAHA.116.023163. PMID 28784826.1000. doi:10.1080/14656566.2017.1333599. PMID 28532183

Stem cells are undifferentiated cells capable-of-proliferation-and differentiation. They can yield a large number of differentiated functional progeny creating the tissue after injury. Stem cell therapy is a modern type of intervention strategy that is introduced-to-treat-a-specific-disease. Renal failure is one of the most common causes of mortality and morbidity-all-over-the-world. Treatment options of chronic renal failure (CRF) mainly involve renal transplantation in addition to dialysis. Kidney transplantation is hampered by shortage of donors while dialysis is encumbered by several limitations-and=-is---associated---with--several-socioeconomic-problems-for-the-patients...........

[1]. Abdel Aziz, M.; Atta, H.; Mahfouz, S.; Fouad, H.; Roshdy, N.; Ahmed, H.; Rashed, L.; Sabry, D.; Hassouna, A. andHasan, N. (2007): Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver cirrhosis. ClinBiochem., 40 (12): 893-899.
[2]. Abdel Gawad, S. and Mohamed, A. (2010):Silymarin Administration Protects Against Cisplatin-Induced Nephrotoxicity in Adult Male Albino Rats. (Histological and Immunohistochemical Study). J. Histol., 33(4): 683 – 691.
[3]. Abdel Meguid, N.; Chmaisse, H. and AbouZeinab, N. (2010): Protective Effect of Silymarin on Cisplatin-induced Nephrotoxicity in Rat. Pakistan Journal of Nutrition, 9 (7): 624-636.
[4]. Ajith, T.;Usha, S. and Nivitha, V. (2007): Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: A comparative study. Clin.Chim.Acta., 375(1-2):82-86. [5]. Al-Kharusi, N.; Babiker, H.; Al-Salam, S.; Waly, M.; Nemmar, A.; Al-Lawati, I.; Yasin, J.; Beegam, S. andAli, B. (2013):Ellagic acid protects against cisplatin-induced nephrotoxicity in rats: a dose-dependent study. Eur Rev Med Pharmacol Sci., 17(3):299-310.

Background: Tuberculosis(TB) is the airborne communicable disease caused by Mycobacterium tuberculi. which has been one of the common disease affecting human communities. Other than disease related complications there are serious ADRs to the ATT. Objectives: The main objectives of study was to evaluate the ADRs induced by ATT drugs and also assess causality and severity and informing to PvPI for signal generation and management was provided. Materials and methods: A study was conducted for 7months at a tertiary care teaching hospital (Mar-Sep) 2019 at SVRRGH hospital Tirupati. Patients who developed at least 1ADR were taken as study subjects. Detection and monitoring of these ADRs was done by interviewing the patients.ADRs were then checked for causality and severity by using Naranjo and Hart wig questionaries respectively...........

Key Words: Adverse drug reactions, clinical pharmacist, NARANJO and HARTWIGSIEGEL scale, Pharmacovigilance, Tuberculosis.

[1]. Amit R.Dedun, Ghanshyam B. Borisagar, Rajesh N.Solanki ,Impact of Adverse drug reaction of fisrt line anti-tuberculous drugs on treatment outcome of tuberculosis under revised national tuberculosis control programme, International journal of advances in medicine ,4(3),2017,645-649.
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[3]. Reena Verma , Mahor GR , Arun Kumar Shrivastava, Prashant Pathak , Advarse drug reactions associated with first-line anti- tubercular drugs in a tertiary care hospital of central india:A study of clinical presentations, causality and severity, Asianj pharm clin res, 7(5),2014,140-143.
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[5]. Sachin Tutu, Surya Kant, Ajay Kumar Verma , Amod kumar Sachan, Rajendra Nath, Narendra Kumar, Arpita Singh and Rakesh Kumar Dixit , Incidence and pattern of adverse drug reactions (ADRs) in patients treated for Tuberculosis under DOTS at a tertiary care hospital of northern india, International journal of pharmaceutical sciences and research, 9(11),2018,4950-4955.