Volume 1 ~ Issue 1
|2||Analgesic and Anti-inflammatory Activity of Roxithromycin and Erythromycin, Alone and in Combination with Ibuprofen: An Animal Study
Akash A Khobragade, Sadiq B Patel, Rupesh R Pophale, Vallish BN, Snehal P Kosale
DOI : 10.9790/3013-01101521
ANED : 05.3013/011015021
Background: Several in-vitro, animal and clinical studies have reported anti-inflammatory activity of macrolides. Anti-inflammatory properties of macrolides form the basis for their experimental use in airway inflammatory and infective conditions like asthma
Materials and Method: After animal ethics committee approval, the anti-inflammatory and analgesic potentials of erythromycin (40 mg/kg) and roxithromycin (20 mg/kg) were evaluated in carrageenan-induced rat paw edema model and rat tail flick model respectively, in comparison and combination with ibuprofen (100 mg/kg) in albino wistar rats.
Results and Interpretation: Both the macrolides were found to have significant anti-inflammatory activity, roxithromycin being slightly more efficacious than erythromycin. Combination with ibuprofen failed to produce improved benefit. Both the macrolides neither showed significant analgesic property alone, nor enhanced analgesic effect of ibuprofen significantly. Conclusion: The macrolides have shown anti-inflammatory activity and a tendency towards analgesic potential; possible mechanisms are discussed. Further studies are required before any recommendations can be made.
Keywords: Analgesic, Animal study, Anti-inflammatory, Erythromycin, Ibuprofen Roxithromycin
|3||Correlation of Hyperglycemia and Succinate dehydrogenase Activity During Hymenolepiasis in Mice and Treatment With Praziquantel
J Parvathi, K Aruna
DOI : 10.9790/3013-01102228
ANED : 05.3013/011022028
Energy metabolism is the chief controlling factor of all living organisms, mostly by the breakdown of glucose. The higher energy demands in a traumatized host directly affects levels of glucose and the enzyme activity involved in its ultimate breakdown. The variations in the carbohydrate levels of host Mus musculus infected with the intestinal helminth Hymenolepis nana and treated with anthelmintic praziquantel, demonstrated histochemically using Periodic acid-schiff reaction, are qualitatively correlated with the corresponding variations of succinate dehydrogenase, a key energy generating enzyme of the Kreb's cycle, visualized by Nitroblue tetrazolium, in the host intestine. These results are also emphasized for the development of prophylactic chemotherapy, especially in the tropical countries, with prevalence of multi-worm infections.
Keywords: Glucose, hymenolepiasis, Nitroblue tetrazolium, Periodic acid-schiff, succinate dehydrogenase
|4||Detection of Diethylene Glycol in Glycerin and Propylene Glycol by using high performance thin layer chromatography HPTLC
M.Ph. Huda Ghanem, Prof. Mhd Ammar Alkhayat, Prof. Mhd Amer Almardini
DOI : 10.9790/3013-01102934
ANED : 05.3013/011029034
We were developed analytical method rapid, sensitive and easy to use to detect Diethylene glycol (DEG) in some of excipients: Glycerin, and propylene glycol by using high-performance thin-layer chromatography HPTLC with plate fluorinated: HPTLC Plate silica gel 60 F 254, and with mobile phase: acetone: toluene: 5 M ammonium hydroxide at rates 85: 5:10, respectively. And Use a scanner with wavelength: 325 nm. This method allows the detection and assay of toxic Diethylene glycol in excipients: glycerin and propylene glycol in concentrations of not less than 0.1%. This method was verified the validity of it to conduct all the constitutional requirements.
Keywords: Diethylene Glycol (DEG), Propylene Glycol (PG), Glycerin, Ethylene Glycol (EG, High Performance Thin Layer Chromatography (HPTLC).
|5||Pharmacodynamic and Pharmacokinetic Drug Interaction of Gliclazide and Olanzapine in Animal Models
Eswar Kumar K, Raghu Ram K, Swathi P, Jyotsna Rani P and Gupta M.N
DOI : 10.9790/3013-01103543
ANED : 05.3013/011035043
Studies were conducted in normal rats, alloxan induced diabetic rats and normal rabbits with oral administration of selected doses of gliclazide, olanzapine and their combination with adequate wash out periods in between treatments. Blood samples were collected from rats and rabbits at regular intervals of time and were analysed for glucose by GOD/POD method and for gliclazide by HPLC method. Glicazide produced hypoglycemia and antihyperglycemia in normal/diabetic rats with peak activity at 1h & 8h and 3h & 10 h respectively and in rabbits at 2 h. Therapeutic dose of olanzapine alone did not alter the normal blood glucose level and it did not alter the hypoglycemic response produced by gliclazide in combination. The same phenomenon observed in diabetic rats and rabbits also. There was no significant change in the serum levels and pharmacokinetic parameters of gliclazide when given in combination with olanzapine in normal rabbits. Hence it is concluded that single dose treatment with olanzapine has no influence on pharmacodynamics/ pharmacokinetics of gliclazide. Since the combination was found to be safe in two dissimilar species, the combination might be safe in humans also.
Keywords: Drug interactions, Gliclazide, Olanzapine, Pharmacodynamics and Pharmacokinetics.