Current-Issue

Volume 7 ~ Issue 6,~Version 1,~June - 2017


Paper Type

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Research Paper

Title

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Development and validation of a simple, fast, isocratic stability indicating RP-HPLC-UV method for the determination of chlorhexidine and its impurity para-chloroaniline in bulk and finished product

Country

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Sudan

Authors

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TarigG. Mohammeda ||, M.EM. Abdel Aziz

Page No.

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01-08

Paper Index
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XXXX
A simple, isocraticRP HPLC-UV method was developed for the simultaneous determination of chlorhexidine (CHD) and p-Chloroaniline (pCA) inchlorhexidine mouth rinses. An excellent separation obtainedbyC18 column (200mm × 4.6 mm, 3μm). Mobile phasewas acetate buffer:methanol in a 45:55 ratio,flowrate was 1.0 ml/min. Both ingredient and an impuritywere detected at 254 nm,injection volume was 20μl and the analysis temperature was room temperature.Resolution4.7,retention times was3.1min and 5.7 min for pCAand CHD respectively. The proposed method was testedforsystem suitability, linearity, range, precision, accuracy, specificity, robustness, detection and quantification limits. The linearity range was40-160μg/ml forCHD and0.3-1.2 μg/ml forpCA.The correlation coefficient of the regression line was 1.000 for both components. Method robustness was tested under nine different conditions using sampleswith a known content. For CHD, the mean of the nine assays was 99.95% andthe RSD was 0.16%. ForpCA, the mean of the nine assays was 99.98% and the RSD was 0.24%. The results show that this is a simple method that can be applied to the analysis of Chlorhexidineproductswith satisfactory degrees of accuracy and precision. Due to the selected optimized conditions, this method can be used with the minimum requirements of an isocratic HPLC system
[1] PaulsonD. S;1993; efficacy evaluation of a 4% chlorhexidine gluconate as a full-body shower wash; American Journal of Infection Control, 21(4), 205-209.
[2] AlbandarJ. M, Rise J, GjermoP andJohansen J. R; 1986; radiographic quantification of alveolar bone level changes; A 2-Year Longitudinal Study in Man; Journal of Clinical Periodontology; 13(3), 195-200.
[3] J. Albandar, Gjermo P, and Preus H;1994; Chlorhexidine use after twodecadesofover-the-counter availability;Journal of Periodontol; 465, 109–112.
[4] Fardal O and Turnbull R.S; 1986; a review of the literature on use of chlorhexidine in dentistry, J Am Dent Assoc;112, 8639.
[5] 5- Leonardo M.R., TanomaruFilho M., Silva L.A.B., Nelson Filho P., Bonifacio K.C; 1999; in vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution; Journal of End Oden;25,167-171.

 

Paper Type

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Research Paper

Title

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Investigating effect of vitamin C on oxidative stress in patients undergoing chronic ambulatory peritoneal dialysis

Country

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Sudan

Authors

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Farshid Padyab

Page No.

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09-11

Paper Index
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XXXX

Oxidative stress increases in chronic renal failure patients and exacerbates during dialysis. The incidence of cardiovascular diseases are high in CRF patients and oxidative stress is a risk factor for CVD in CRF patients. The aim of this study was to assess the effect of antioxidant therapy including vitamin C on two markers of oxidative stress including MDA and TAC in patients undergoing peritoneal dialysis (PD). Materials and methods: This is a prospective clinical trial conducted on 40 patients undergoing PD and were randomly divided into two groups of intervention (n=20) and placebo (n=20). The intervention group received 250 mg/day vitamin Corally and 250 mg/day vitamin C orally for 8 weeks. Placebo group received placebo which was similar with intervention drug in terms of shape and color. The age, sex, CRP, albumin, TG, HDL, LDL, uric acid were measured at baseline. The serum and peritoneal MDA and TAC levels were measured at baseline and at the end of the study................

 

Keywords: Peritoneal dialysis, oxidative stress, TAC, MDA, serum

[1]. Montazerifar F, Karajibani M, Sanadgol H, Hashemi M. Effect of peritoneal dialysis on antioxidant defense system and oxidative stress. Hong Kong Journal of Nephrology. 2012 10//;14(2):33-7.
[2]. Sundl I, Roob JM, Meinitzer A, Tiran B, Khoschsorur G, Haditsch B, et al. Antioxidant status of patients on peritoneal dialysis: associations with inflammation and glycoxidative stress. Peritoneal Dialysis International. 2009;29(1):89-101.
[3]. Gokal R, Mallick N. Peritoneal dialysis. The Lancet. 1999;353(9155):823-8.
[4]. Kamgar M, Zaldivar F, Vaziri ND, Pahl MV. Antioxidant therapy does not ameliorate oxidative stress and inflammation in patients with end-stage renal disease. Journal of the National Medical Association. 2009;101(4):336-44.
[5]. Daschner M, Lenhartz H, Bötticher D, Schaefer F, Wollschläger M, Mehls O, et al. Influence of dialysis on plasma lipid peroxidation products and antioxidant levels. Kidney international. 1996;50(4):1268-72.