Volume 3 ~ Issue 1 (Version 2)

 

 

Paper Type

::

Research Paper

Title

::

Correlation Of Body Composition With Dynamic Lung Function.

Country

::

India

Authors

::

Dr.SwikrutiBehera||Dr.Bipinbihari pradhan

Page No.

::

01-03

Paper Index

::

DOI : 10.9790/3013-312013  

ANED :: DOI :05.3013/03120103

Objectives: Abstract:Lung volumes that depend upon the rate at which air flows out of the lungs are termed dynamic lung volume. Dynamic lung volumes include Forced Vital Capacity(FVC), forced Expiratory Volume in 1 Second(FEV1)& FEV1/FVC ratio. These lung parameters are very commonly used in clinical practice to diagnose various obstructive and restrictive diseases. For long, Body Mass Index & Waist Hip Ratio are being used to assess the obesity and their effect on lung function. But BMI has the limitation of not distinguishing between fat and muscle mass. So this study was undertaken to study that which body parameter is responsible for improved or worsened lung function.150 healthy medical students(85 males and 65 females) with age group 18-24 years were included in the study. Their body composition was studied using 'Bioelectric Impedance' method. Each dynamic lung function was correlated with body fat percentage (BF%), fat free mass(FFM), fat free mass index(FFMI), BMI & waist hip ratio. FFM showed highest as well as significant correlation with FVC, FEV1& FEV1/FVC.BF% has insignificant correlation with all dynamic lung function. BMI & W/H ratio, have lower or insignificant correlation with dynamic lung functions, than FFM & FFMI.

 

Keywords: Dynamic lung volumes, Forced vital capacity, Forced expiratory volume, Body mass index, Waist hip ratio, Body fat percentage, Fat free mass, Fat free mass index.

[1]. Consolzia, Toni, Pelona, 1963 .Physiological Measurements of Metabolic Function in Man.P-225
[2]. Jones RL, Magdalene M, Nzekwu U. Effects of Body Mass Index on Lung Volume. Chest 2006;130:827-833
[3]. Cotes JE, Chinn DJ, Miller MR. Lung Function, Physiology, Measurement and application in Medicine, Blackwell,6th Edition: 2006 ; 37-39
[4]. Ray CS, Sue DY, Bray G, Hansen JE, Wasserman K. Effect of Obesity on Respiratory Function. Am Review Resp Disease ;1983 :128,501-506
[5]. Spengler CM, Cotes SA. Endogenous Circadian Rhythm of Pulmonary Function in healthy humans. Am J RespirCrit Care Med, 2002 ;166: 1005.
[6]. Brusasco V, Crapo R, Viegi G. General consideration for Lung Function Testing. ATS/ERS Task force: Standardisation of Lung function test; EurRespir J. 2005; 26: 153-161
[7]. Young JP, Pach JP, Jung H The effect of Body mass index, fat percentage and fat free mass Index on pulmonary function test – with particular reference to parameters derived from forced expiratory volume curve :Tub &Resp Dis ; 2003 : 54 (2) : 210-218
[8]. Muralidhara DV & Ramesh M. Some aspects of pulmonary functions in the underweight & overweight human subjects . The journal of physiological sciencesVol – 20, 2007
[9]. Helena Z, Emaneula T, Paolo T L B, Manio , Havio B, Manno Z, Relation between body composition, fat distribution & lung function in elderly man. Am J ClinNutr 2001; 73 827-31. Chen Y, Horne SL, Dosman JA Body weight & weight gain related to pulmonary function decline in adults: a six year follow up study. Thorax 1993 ; 48 : 375-380.
[10]. Muls E, Vryens C, Michols A. The effects of abdominal fat distribution measured by computed tomognaphy on the Resp. System in non-smoking obese women Int J obes 1990 :14(Supp 2):136
[11]. Jenkins SC, Moxaham J. The Effect of mild Obesity on Resp Disease 1991; 85; 309-11.
[12]. Pullicino E, Coward W, Stubbs RJ, et al. Bedside and field methods for assessing Body Composition : comparison with the deuterium dilution technique. Eur J ClinNutr 1990; 44:753–62
[13]. Khan M, O‟ Hara, Pohlman RL, Goldstein DG, Guha SK. Multidimensional applications of Bioelectrical Impedance analysis; JEPonline: 2005; 8 (1): 56-71.

 

Paper Type

::

Research Paper

Title

::

Resveratrol And Its Derivative Improves Oxidative Stress and Protects Against Alloxan- Induced Diabetics

Country

::

Iraq

Authors

::

Montaha A. Al-Safar

Page No.

::

.04-09

Paper Index

::

DOI : 10.9790/3013-312049  

ANED :: DOI : 05.3013/03120409

Objectives: Resveratrol is a non flavonoid polyphenol and it has three phenolic hydroxyl groups and shown to have its biological effects . Reactive oxygen species play a crucial role in the pathogens of diabetes and its complication, this study aims to examine the effects of resveratrol and its derivative on alloxan induced diabetics. Statistical analysis showed a significant decrease in SOD , GSH, Gpx, Zn and Cu concentration and significant increase in MDA level on diabetic rabbits p<0.05 , however diet supplemented with resveratrol derivative induced a significant increase of copper and zinc 1.43±0.01 ppm, 0.79 ± 0.02 ppm respectively after sixth weeks of treatment as compared with control 1.13 ± 0.03ppm, 0.66 ± 0.01ppm respectively. . The administration of resveratrol derivative to rabbits resulted in the significant decrease of MDA levels after three weeks to 2.42±0.08 nanomole /ml and 1.56±0.02 nanomole/ml after sixth weeks as compared with control group 2.43 ± 0.2778 nanomole /ml. The diabetic rabbits presented a significant increase in the SOD activity after treatment by resveratrol derivative after sixth weeks 4.24 ± 0.02 U/ml as compared with control during the experimental period, glbcimide treated group show a significant decrease in SOD activity after sixth weeks . In addition, there was a significant increase in Gpx activity119.77±0.01 U/L in resveratrol derivative treated group after sixth weeks. In infected animals was a decrease level of GSH when drug and purified resveratrol treated rabbits, while resveratrol derivative has normal value after three weeks of treatment 11.17 ± 0.03 μ mole/L and 19.82 ±0.01 μ mole/L after sixth weeks. The results suggest thatresveratrol derivative may be helpful in preventing diabetic complications in rabbits.

 

Keywords: antioxidant enzymes, diabetic disease , oxidative stress , resveratrol.

[1]. Tomas-barberan, F.A. and Robins, R.J.(1997).Phytochemistry of fruit and vegetables.(Clarerdon press- Oxford. New York).
[2]. Ungvoeri Z, Orosz Z. and Rivera A (2007).Resveratrol increase vascular oxidative stress resistance .Am.Physiol.Heart Circ Physio. 292.
[3]. Mattivi F, Vrhovsek U, Malacarne G, Masuero D, Zulini L, Stefanini M, Moser C, Velsco R. and Guella G (2011). Profiling of resveratrol oligomers, important stress metabolites, accumulating in the leaves of hybrid Vitis vinifera (Merzling-Teroldego) genotypes infected with plasmopara viticola. Agric Chem 59, 5364–5375.
[4]. Rukmini M S, Benedicta D D. and Uivian D. (2004) .Superoxide dismutase and catalase activities and their correlation with malonaldehyde in schizophrenic patients. Ind Clin Biochem 19, 114-118.
[5]. Ungvari Z and Csiszar A.(2011) .Resveratrol confers endothelial prodection in insulin – dependent diabetes mellitus.Cardiovasc. Drugs Ther 25, 111-113.
[6]. Sekeroglu M R, Sahin H, Dulger H. and Algun A. (2004) .The effect of dietary caloric restriction on antioxidant status and lipid peroxidation in mild and sever streptozotocin- induced diabetic rats.Clin Chem Acta 348, 121-129.
[7]. Silan C. (2008).The effect of chronic resveratrol treatment on vascular responsiveness streptozotocin – induced diabetic rats. Bio Pharm Bull 31, 897-902.
[8] Fendri C, Mechri A, Khiari G,Othman A, Kerkeni A. and Gaha L. (2006).Oxidative stress involvement in schizophrenia pathophysiology . Enephalo. 32, 244-252.
[9]. Mates J.M. and Sanchez –Jimenez F(1999).Antioxidant enzymes and their implications in path physiologic processes. Front Biosci .15, 339-345.
[10]. Mates JM, Perez-Gomez C. and Nanez de Castro I (1999).Antioxidant enzymes and human diseases. Clin. Biochem 32, 595-603.
[11] Louise AB, Cathrine JK, Paul W, Nicholas F, Jane M and Tony A. (2009).Oxidative stress regulates a dipocyte apolipoprotein E and suppresses its expression in obesity .J. Diabetes 57,2992-2998.
[12]. Keaney JFJr, Larson MG, Vasan RS and Wilson PWF (2003).Obesity and systemic oxidative stress :clinical correlates of oxidative stress in the Framingham study . Arterioscler. Thromb, Vasc.Biol.23, 434-439.
[13]. Shah PK and Patel J.A. (2010) Resveratrol and its biological actions .Review article .4(1):15-21.
[14] Harbone JB. (1984). Phytochemical methods, chapman and Hall London.
[15]. Al-Jumaily EF, Al-bayati RA and Shafiq ZA (2012). Antidiabetic and antihyperglycemia effects of Resvertrol and its Derivative on Alloxan diabetic rabbits. IOSR J of Pharmacy Vol.2, 50-58.

 

 

Paper Type

::

Research Paper

Title

::

Thermal, X-Ray Diffraction, Spectral and Antimicrobial Activity of Bivalent Metal (Zn, Cd, Hg, Pb and Ag) Chelates Of 1, 2 Naphthoquinone – 2, Oxime,

Country

::

India

Authors

::

N. R. Gonewar,||V. B. Jadhav,||S.S. Sakure ||K. D. Jadhav|| R. G. Sarawadekar

Page No.

::

10-17

Paper Index

::

DOI : 10.9790/3013-31201017  

ANED :: DOI : 05.3013/0312010017

Objectives: Five metal chelates of the type M [NQO] 2 where M = Hg, Cd, Zn, Pb: NQO = 1, 2 napthoquinone – 2, oxime and Ag (NQO) have been synthesized. All chelates have been characterized by modern methods such as elemental analysis, FTIR, Electronic spectra. 1H & 13C NMR, Thermogravimetry, Differential scanning calorimetry and electron microscopy with EDAX analysis of chelates were carried out. All chelates are found to be coloured and mercury, lead, zinc and cadmium chelates are octahedral while silver chelate has been assigned square planer. These chelates are thermally stable up to 7000C and all are crystalline in nature. Their particle sizes are in the range of 30-50 nm. The ligand and the metal chelates have been screened for antimicrobial activity on gram positive and gram negative bacteria and fungi and the results are compared with cisplatin as standard.

 

Keywords: 1-2 naphthoquinone -2, oxime, X-ray diffraction, IR, NMR, SEM, Antimicrobial activity, Electronic spectra

[1]. A.P. Avdeenko, G.M. Glinyanaya and V.V. Pirozhenko, Russian Journal of Organic Chemistry, 35(10)1480-1487 (1999).
[2]. B. Foretic and N. Burger, Monatshefte Fur Chemie, 127, 227-230 (1996).
[3]. P. Lingaiah and E. V. Sundaram, Current Science Vol. 45, N0.2, 51-52 (1976).
[4]. S. Gurriari and G. Siracus, Inorganica Chimica Acta., Vol. 5 No.3, 650-654 (1971).
[5]. R.G. Sarawadekar and I.B. Gade, Software for Kinetic Analysis of non isothermal thermogravimetry (TG) Data, HEMRL REPORT , PYRO, TR 253 (2006).
[6]. Le Bail, A., Powder Diffraction, 19,249-259 (2002).
[7]. A.Buraway, M. Cais, J. T Chamberlain, F. Livesedge and A. R. Thompson, J. Chem. Soc 3277 (1955).
[8]. N.R. Gonewar, V. B. Jadhav K.D. Jadhav, and R.G. Sarawadekar, Research in Pharmacy 2(1) : 18-25, 2012
[9]. Constantino V. R. Toma H. E., Olivenia LFC and P. J. Raman Sepctroscopy, 23, 629 (1992).
[10]. Natarajan C., Nazeer Hussain A. Indian J. Chem. 22A, 527 (1983).
[11]. A. Krzan and J. Mavri., Chemical Physics, 277, 71-76 (2002).
[12]. V. Enchev, G. Ivanova, A. Vgrinov, G. D. Neykov., J. Mol, Struct. 508, 149 (1999).
[13]. G. Ivanov, V. Enchev. Chem... Phys. 264, 235 (2001).
[14]. T. Shono, Y. Hayashi and K. Shinra., Bull. Chem. Soc. of Japan., 4, 3179-3181 (1971).
[15]. Z.H. Chohan, C. T. Superan and A. Scozafava, J. Enzyme Inhihitian and Medicinal Chemistry, 20, 303 (2005).

 

Paper Type

::

Research Paper

Title

::

IIn Vitro CYTOTOXIC STUDY FOR PARTIAL PURE LIGNAN EXTRACT FROM FLAX SEED (Linum usitatissirnurn) ON RHABDOMYOSARRCOMA (RD) AND MURINE MAMMARY ADENOCARCINOMA (MMA) CELL LINES

Country

::

Iraq

Authors

::

Essam F. Al-Jumaily||Auday A. Mahdi

Page No.

::

18-24

Paper Index

::

DOI : 10.9790/3013-31201824  

ANED :: DOI : 05.3013/0312018024

Objectives: The lignan (Secoisolaricinesinal diglucoside, SDG), was obtained from flaxseed ( Linum usitatissirnurn) appear to act as both anti-estrogens and weak estrogens. The anti-estrogenic potential of lignans has been postulated to protect against hormone sensitive cancers such as breast, prostate and colon cancer. The aim of this study was to examine the cytotoxic effect of partial pure lignan on some cell lines including, the Murine Mammary Adenocarcinoma, Rhabdomyosarcoma RD, L20B and normal cell line (Rat Embryo Fibroblast REF). Four concentrations of partial pure SDG which obtained by using alcohol hydrolysis dioxin / ethanol flaxseed extraction and liquid/liquid partition, have been prepared 5, 10, 15, 20 μM with different of exposure periods 24, 48, 72 h. These parameters have been applied on all cancer and normal cell line. The highest percentage of inhibition appears in 5, 10,15 μM at 24 h (60.11, 49.99, 50.28 %) respectively on RD cell line when treated with partial pure. and the with same concentrations this percentage decreases without any significant difference. In case of L20B cell line in 72 h, the inhibitory effect of partial pure at 5,10 μM, 57.67 and 52.11 % respectively. While the cytotoxicity of partial pure SDG on ANM3 cell line appeared in 24h at 15um which was 64.88%. The lignans are growth inhibitors of colon tumor cells and suggest act through mechanisms other than anti-estrogenic activity.

Keywords: Lignan (Secoisolaricinesinal diglucoside), matairesinol, Murine mammary adenocarcinoma, Rhabdomyosarcoma RD, L20B cell lines, Inhibitory effect, growth inhibitors.

[1]. Thompson, LU.; Robb, P.; Serraino, M.and Cheung, F. (1991) Marnmalian lignan production from various foods. Nutr Cancer 16:43-52.
[2]. Serraino,M. and Thornpson, L.U. (1992).The effect of flaxseed supplementation on the initiation and promotional stages of mammary tumorigenesis.Nutr Cancer 17: 153-159.
[3]. Thompson, L.U.; Li, T.; Chen, J. and Goss, P.E.(2000).Biological effects of dietary Flaxseed in patients with Breast Cancer Research and Treatment.
[4]. Demark-Wahnefried, W.; Price, D.T.; Polascik, T.J.; Robertson, C.N.; Anderson, E.E.; Paulson, D.F.; Walther, P.J.; Gannon, M. and Vollmer, R.T. (2001).Pilot study of dietary fat restriction and flaxseed supplementation in men with prostate cancer before surgery: exploring the effects on hormonal levels, prostate-specific antigen, and histopathologic features. Urology.58:47–52.
[5]. Rickard, S.E.; Orcheson, L.J.; Seidl, M.M.; Luyengi, L.; Fong, H.H.S. and Thompson, L.U. (1996). Dose dependent production of mammalian lignans in rats and in vitro from the purified precursor secoisoIariciresinol diglycoside in flaxseed. J Nutr l26:20I2-20lg.
[6]. Mahdi, A.A. (2007). Extraction and Purification of Lignan and study of its effect on cancer cell in vitro. MSc. Thesis. Genetic Engineering and Biotechnology for post graduate studies, Baghdad Universitry.
[7]. Westcott, N.D. and Muir, A.D. (1997).Medicinal Lignan from flaxseed. MAP Conference.
[8]. Freshney, R.I. (2000). Culture of animal cells: A manual for basic technique (4thed.). Wiley-liss, A John wiley and sons, Inc. publication, New york.
[9]. Thompson, L.U.; Seid, M.M.; Rickard, S.I.; Orcheson, L. and Fong, H.H.S. (1996). Antitumorigenic effect of a rnammalian lignan precursor from flaxseed. Nutr Cancer 26: 159-165.
[10]. SPSS Base 10.0. (1998). User's Guide. Chicago: SPSS Inc.

 

Paper Type

::

Research Paper

Title

::

Assessment Of The Bacteriological Quality Of Ice Cream Offered For Public Consumption In Bauchi. Nigeria

Country

::

Malaysia,

Authors

::

Moshood A. Yusuf, ||TengkuHaziyamin Abdul ||Tengku Abdul Hamid||,Mr. Moshood A. Yusuf

Page No.

::

25-30

Paper Index

::

DOI : 10.9790/3013-31202530  

ANED :: DOI : 05.3013/0312025030

30 samples of locally produced and industrially produced ice cream were examined for the presence of bacteria contaminants. Bacteria count was carried out on the different samples using pour plate method. Isolates were identified using standard procedure. Different types of bacteria isolated include Bacillus cereus, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Salmonella typhi. The total bacteria count per ml of the locally produced ice cream sample range from 1.44 – 3.0 4 and the total bacteria count per ml of the industrially produced ice cream sample range between 3.03 - 8.83cfu/ml. The mean count of locally produced and industrially produced ice cream was 2.04 and 5.33 respectively, therefore industrially produced ice cream is more hygienic than locally produced ice cream.The presence of possible pathogenic organisms in the analyzed ice-cream sample should be viewed with concern by the consumers, producing company and the Government since food poisoning by Staphylococcus sp, Bacillus sp and Salmonella sp is possible through consumption of contaminated ice-cream.

 

Keywords:- ice cream, mean count, pathogenic organisms

[1]. Adams and Moss, Food microbiology, the Royal society of chemistry. Thomas Graham house, Science Park Cambridge UK 1999, 78-106.
[2]. A.E. MalouAduli, and E. Uzochukwu, Yoghurt production for small scale milk processor. NAPRI Bulletin June 1999 Vol. 2, 273.
[3]. S. Akinuli, (1998), Yoghurt drinks manufacturing. Business guide. National Concord May 22nd, 1998, .22 .S.A.Farrag, and, E.H. Marth, 'Interactions between Listeria monocytogenes and other psychotropic bacteria in dairy foods: A review', Food Australia, 44 (1992), 281–286.
[4]. R.T.Marshall, and W.S. Arbuckle, Ice Cream. 5thed. Chapman & Hall, New York 1996..
[5]. R.A. Warke, M. Kamat, P. Kamat, P. Thomas, 'Incidence of pathogenic psychrotrophs in ice creams sold in some retail outlets in Mumbai, India' Food Control, 11 (2000), 77–83.
[6]. C.P. Champagne, R.R. Laing, D. Roy, A.A. Mafu, 'Psycrotrophs in dairy products: Their effects and their control', Crit. Rev. Food Sci. Nutr., 34, 1994, 1–30.
[7]. N.A Daniels, L. Mackinnon, S.M. Rowe, N.H. Bean, P.M. Griffin, P.S. Mead, 'Foodborne disease outbreaks in United States schools', Pediatr. Infect. Dis. J., 21, 2002, 623–628.
[8]. K. Chug, 'Salmonella outbreak from ice cream,' Indian Pediatr., 33, 1996, 976–977.
[9]. T. Djuretic, P. Wall, G. Nichols, 'General outbreaks of infectious intestinal disease associated with milk and dairy products in England and Wales: 1992 to 1996', Commun. Dis. Rep. Rev. CDR Rev., 7, 1997, R41–R45.
[10]. M. Digrak, S. Ozcelik, 'The microbiological quality of ice cream sold in Elazig city', Food, 16, 1991, 195–200.

 

Paper Type

::

Research Paper

Title

::

Synthesis And Characterization Of Nano Structured (4- Aminopyridine) Copper (Ii) Acetate Chloride

Country

::

India

Authors

::

A. Sinthiya|| Babu Varghese

Page No.

::

31-36

Paper Index

::

DOI : 10.9790/3013-31203136  

ANED :: DOI : 05.3013/0312031036

The greenish blue [C5H6N2]Cu[C4H6O4]Cl2 particles have been prepared at the room temperature. The grown particles were observed using high-resolution transmission electron microscopy-HRTEM. The results show that, the as-grown particles have the nano tube like structure with breadth 70.9 nm size, and have the structure of 4-aminopyridine copper II acetate chloride. Using Scherrer equation the calculated crystallite size is 0.0325 nm for K=1 and crystallite size is 0.0293 nm for K= 0.9. Using Williamson and Hall theories the calculated ΔK(nm-1) from FWHM is tabulated. From scatter plot we could identify that the more points strongly tend to cluster around a straight line, and it ensures that there is a linear relationship between the two variables (the higher the correlation). Moreover the line around which the points tends to cluster runs from lower left to upper right, so the relationship between the two variable is positive (direct).The elemental analysis conforms that it has 28.01 % of carbon, 2.73 % of hydrogen , 7.01 % of nitrogen, 21.45 % of oxygen, 18.64 % of copper and 19.08 % of chloride. The calculated molecular mass of the as - grown nano tube like structured particle is 346.65 g/mol

 

Keywords:- Fampyridine, acetate, copper chloride, nano tube, HRTEM,Scherrer formula, Williamson and Hall plot

[1]. Zayat et al., 2003, A new strategy for neurochemical photodelivery, Metal-ligand heterolytic cleavage, J. Am. Chem. Soc., 125: 882-883.
[2]. Baxter, P.N.W., Sauvage, T.P., Hosseini, M.W., 1996, Comprehensive Supramolecular Chemistry, Metal Ion Directed Assembly of complex Molecular Architectures and Nanostructures, Pergamon, Oxford,, 165-211.
[3]. Amrehein, Wash, P.L., Shivanyuk, A., Rebek, 2002. J. Org. Letters,4: 319-32.
[4]. Ikeda, A., Udzu, H., Yashimura, M., Shinkai, S., 2000.Tetrahedron, 56:1825-1832.
[5]. Taccola, G. and Nistri,2005. A., Acta Neurochir, 93 : 151-154.
[6]. .P. Pohanish, Stanley A, 2009. Wiley guide to chemical incompatibilities, Greene, John Wiley and sons, second edition, P 64.
[7]. Jude S. and Bever C.,2006. Pharmacol.,111 : 224 – 259.
[8]. Strupp, M., Malla, R., Dichgan, M., Fraitinger, T., Glasaner, S. and Brandt, 2004. T., Neurology, 62 : 1623-1625.
[9]. Schwid, S. B., Petrie, M. D., Mc Dermott, M.P, Tierney. D.S., Mason. D.H and Goodman A.D,1997. Neurology, 48: 817-821.
[10]. Andersonn et al., 2005. Actacryst, E61 : o1350-o1353.
[11]. Chao, M. and Schempp. E., 1977. Acta Cryst., B33:1557-1564.
[12]. Sinthiya, A., Samuel Robinson Jebas and Suganthi Devadasan, 2008. Acta Cryst..E64 : m853-m854.
[13]. Michael B. Kerber et al., 2005. Rev.Adv.Mater.Sci., 10: 427-433.

 

Paper Type

::

Research Paper

Title

::

Effect Of Pgf2α On Conception Rate In Suboestrus Dairy Buffaloes

Country

::

INDIA

Authors

::

L. Ramsingh|| K.Muralimohan

Page No.

::

37

Paper Index

::

DOI : 10.9790/3013-312037  

ANED :: DOI : 05.3013/0312037037

Suboestrus is the major causes of infertility in post partum buffaloes which leads to economic losses due to increased service period. The present study was undertaken to evaluate efficacy of PGF2αin suboestrus dairy buffaloes.

 

Keywords: PGF2α, Dairy buffaloes, Suboestrus

[1]. Gautam, A.P., Kharche, K.G. and Thakur, M.S. (1990)…. Indian J. Anim. Repord. 11:164
[2]. Pant, H.C. and Singh, B.P. (1991)…. ibid., 12:55
[3]. Singh, G.B., Dugwekar, Y.G., Sharma, R.D., Chavan, F.S. and Singh, M. (1979)…. Vet. Record. 104:412

 

Paper Type

::

Research Paper

Title

::

Mukkuddu Maathrai (Triple Tablet), An Indigenous Medicine Elevates Serum Prolactin Level Of Female Rats (Rattus norvegicus)

Country

::

Sri Lanka

Authors

::

Kanisra Mary F|| Eswaramohan T||Surendran S N||Murugananthan A||Romakeswaran P

Page No.

::

38-42

Paper Index

::

DOI : 10.9790/3013-31203842  

ANED :: DOI : 05.3013/031203804

The Mukkuddu Maathrai is a mixture of Kasturi, Saffron and Korosanai. Ayurvedic doctors in Jaffna peninsula have been prescribing this mixture to patients for their good health. The prolactin (PRL) can be used as a marker in hypothalamo-pituitary axis. The objective of the present study was to determine the effect of Mukkuddu Maathrai treatment on serum prolactin level of female rats at estrus stage. Female rats were maintained under uniform conditions. Stages of oestrus cycle were determined by using vaginal smear cytology. Initial blood sample was collected by tail bleeding at the estrus stage and were treated with oral feeding. Tests and controls were fed with 5% w/v of the Mukkuddu Maathrai mixture solution and distilled water alone respectively. Treatments were done continuously as once per day until one oestrus cycle complete. At the next estrus stage, second blood sampling was performed. Serum PRL levels were measured by enzyme immunoassay and the results were compared by using paired't' test at 95% confident limits. All experiments were conducted with their duplicates. During the treatment, prolactin secretion increased significantly in female rats at estrus stage. Mukkuddu Maathrai treatment triggers hypothalamo-pituitary axis of female rats and thus influences on serum prolactin level.

 

Keywords:- Estrus, Kasturi, Korosanai, Prolactin, Saffron..

[1]. C.S. Negi, V.S. Palyal, Traditional Uses of Animal and Animal Products in Medicine and Rituals by the Shoka Tribes of District Pithoragarh Uttaranchal, India, Ethno-Medicine, 1(1), 2007, 47-54.
[2]. M.N. Shrestha, Animal welfare in the musk deer, Applied animal behaviour Science, 59, 1998, 245-250.
[3]. F.I. Abdullaev, Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L), Experimental Biology and Medicine, 227, 2002, 20-25.
[4]. K.M. Nadkarni, and A.K. Nadkarni, Dr K.M. Nadkarni's Indian Materia Medica,1 (Bombay Popular Prakashan, 2002).
[5]. T.V. Sambasivampillai, Dictionary of Medicine, Chemistry, Botany and Allied Sciences, 2 (Directorate of Indian medicine and Homoeopathy, Madras-600106, 1991).
[6]. C Bole-Feysot, V Goffin, M Edery, N Binart, and P.A. Kelly, Prolactin (PRL) and its receptor: actions signal transduction pathways and phenotypes observed in PRL receptor knockout mice, Endocrinology Review, 19, 1998, 225-268.
[7]. R.A. Frost, J Mazella, and L Tseng, Insulin like growth factor binding protein-1 inhibits the mitogenic effect of insulin like growth factors and progestins in human endometrial cells, Biology of Reproduction, 49, 1993, 104-111.
[8]. J Weiss, G.R. Taylor, F Zimmermnn, and K Nebendhl, Collection of body fluids, in C.W. Hume (Eds), The UFAW Handbook on the Care and Management of Laboratory Animals (Churchill Livingstone, Edinburgh London and New York, 2000) 485-493.
[9]. M.E. Freeman, The ovarian cycle of the rat, in E Knobil and J. Neil (eds.), Physiology of reproduction (Raven Press Ltd, New York, 1988) 1893-1928.
[10]. S.J. Singletary, A.J. Kirsch, J Watson, B.O. Karim, D.L. Huso, P.D. Hurn, and S.J. Murphy, Lack of Correlation of Vaginal Impedance Measurements with Hormone Levels in the Rat, American Association for Laboratory Animal Science, 44(6), 2005, 37-42.
[11]. C.J. Reburn, and K.E.W. Edwards, Novel Patterns of Progesterone and Prolactin in Plasma during the Estrous Cycle in the Djungarian Hamster (Phodopus campbelli) as Determined by Repeated Sampling of Individual Females, Biology of Reproduction, 54, 1996, 819-825.
[12]. H Shivalingappa, N.D. Satyanaranyan, M.G. Purohit, A Sahranabasappa, and S.B. Patil, Effect of ethanol extract of Rivea hypocraterifomis on the oestrus cycle of the rat, Journal of Ethnopharmacology, 82, 2002, 11-17.
[13]. M.E. Freeman, B Kanyicska, A Lerant, and G Nagy, Prolactin: Structure, Function, and Regulation of Secretion, Physiological Review, 80(4), 2000, 1523-1631.
[14]. G.N. Babu, and E Vijayan, Plasma gonadotropin, prolactin levels and hypothalamic tyrosine hydroxylase activity in rats during estrous cycle, after overiectomy and after blockade of catecholamine biosynthesis, Biosci, 5(2), 1983, 139-145.
[15]. Y Amenomori, C.L. Chen, and J Meites, Serum Prolactin Levels in Rats during Different Reproductive States, Endocrinology, 86(3), 1970, 506-510.

 

Paper Type

::

Research Paper

Title

::

In Vitro studies on changes in selected biochemical parameters and morphology of Sertoli cells in mice overexpressing bovine growth hormone

Country

::

India

Authors

::

M. Bhaskar

Page No.

::

43-48

Paper Index

::

DOI : 10.9790/3013-31204348  

ANED :: DOI : 05.3013/0312043048

The aim of the present study was to investigate the changes in selected biochemical variables in Sertoli cells isolated from testis of mice (PEPCK-bGH) overexpressing growth hormone. Significant increase in the levels of total proteins (108.33%; p<0.001), lactic acid (95.23%; p<0.001), DNA (155.42%; p<0.001) and RNA (90.00%; p<0.001) were observed in transgenic Sertoli cells as compared to normal Sertoli cells. Further, cultured transgenic Sertoli cells exhibited distinct nucleus over a period of 5 days and on day 7, exhibited accelerated proliferation as compared to normal Sertoli cells. From the results, it can be concluded that growth hormone at least in part mediates modulation of selected biochemical variables to influence Sertoli cell morphology thereby spermatogenesis in mice.

 

Keywords: DNA, lactic acid, PEPCK-bGH transgenic mice, RNA, Sertoli cells, total proteins

[1]. N. Magon, S. Singh, A. Saxena and R. Sahay, Growth hormone in male infertility, Indian Journal of Endocrinology and Metabolism 15, 2011, 248-249.
[2]. A. Bartke, V. Chandrashekar and R.W. Steger, Effects of growth hormone on neuroendocrine function, Acta Neurobiologiae Experimentalis 56, 1996, 833-842.
[3]. D. Madhukar and S. Rajender, Hormonal treatment of male infertility: Promises and pitfalls, Journal of Andrology 30, 2009, 95-112.
[4]. Z. Shoham, G.S. Conway, H. Ostergaard, N. Lahlou, P. Bouchard and H.S. Jacobs, Cotreatment with growth hormone for induction of spermatogenesis in patients with hypogonadotropic hypogonadism, Fertility and Sterility 57, 1992, 1044-1051.
[5]. C. Carani, A.R. Granata, M. De Rosa, C. Garau, S. Zarrilli, L. Paesano, A. Colao, P. Marrama and G. Lombardi, The effect of chronic treatment with GH on gonadal function in men with isolated GH deficiency, European Journal of Endocrinology 140, 1999, 224-230.
[6]. J.M.S. Barlett, H.M. Charlton, I.C.A.F. Robison and E. Nieschlag, Pubertal development and testicular function in the GH-deficient rat. Journal of Endocrinology 126, 1990, 193-201.
[7]. S. Kalra, B. Kalra and A. Sharma, Growth hormone improves semen volume, sperm count and motility in men with idiopathic normogonadotropic infertility, Endocrinology Abstracts 16, 2008, 613.
[8]. Z.J. Champion, M.H. Vickers, C.G. Gravance, B.H. Breier and P.J. Casey, Growth hormone or insulin-like growth factor-I extends longevity of equine spermatozoa in vitro, Theriogenology 57, 2002, 1793-1800.
[9]. M.G. Castro, Transgenic models in endocrinology, (USA: Kluwer Academic Publishers, 2001).
[10]. A. Bartke, E.M. Naar, L. Johnson, M.R. May, M. Cecim, J.S. Yun and T.E. Wagner, Effects of expression of human or bovine growth hormone genes on sperm production and male reproductive performance in four lines of transgenic mice, Journal of Reproduction and Fertility 95, 1992, 109-118.

 

Paper Type

::

Research Paper

Title

::

Effect of Silver Nanoparticles On Energy Metabolism In Selected Tissues Of Aeromonas Hydrophila Infected Indian Major Carp, Catla Catla

Country

::

India

Authors

::

T. Kiran Reddy,||S. Janardana Reddy ||T.N.V.K.V. Prasad

Page No.

::

49-55

Paper Index

::

DOI : 10.9790/3013-31204955  

ANED :: DOI : 05.3013/0312049055

The aim of the present study was to evaluate the effect of silver nanoparticles (AgNPs) on energy metabolic enzymes in selected tissues of Aeromonas hydrophila injected Indian major carp, Catla catla. Fishes exposed to 1/10th concentration of AgNPs (100 μg/l) did not alter lipid peroxidation levels as compared to 1/5th concentration of AgNPs in gills, liver and kidney of fishes. Therefore, 1/10th concentration of AgNPs was selected for subsequent experiments. Significant decrease in the activity levels of succinate dehydrogenase with a significant increase in the activity levels of glucose-6-phosphate dehydrogenase and lactate dehydrogenase in gills, liver and kidney of A. hydrophila infested fishes as compared to controls over a 14-day exposure period. On the other hand, exposure of bacterial infected fishes to 1/10th concentration of AgNPs reversed the activity levels of enzymes in selected tissues as compared to bacterial infected fishes. However, no significant changes were observed in the activity levels of selected enzymes in tissues of fishes exposed to 1/10th concentration of AgNPs alone over controls. From the results, it can be concluded that A. hydrophila injection leads to prevalence of anaerobic conditions to meet the demand of energy in tissues whereas AgNPs through their antibacterial properties mitigate A. hydrophila induced changes in oxidative metabolism thereby energy crisis in tissues of fishes.

 

Keywords:- A. hydrophila, Catla catla, lipid peroxidation, oxidative enzymes, silver nanoparticles

[1]. K. Anjani, P.K. Joshi and S.B. Pratap, Fisheries Sector in India: An Overview of Performance, Policies and Programmes, in K. Anjani, K.K. Pradeep and P.K. Joshi (Eds.), A Profile of People, Technologies and Policies in Fisheries Sector in India, (2003) 1-16. [2]. S. Ayyappan, and J.K. Jena, Grow-out production of carps in India, Journal of Applied Aquaculture 13(3/4), 2003, 251-282. [3]. B. Mohanty, S. Mohanty, J. Sahoo and A. Sharma, Climate Change: Impacts on Fisheries and Aquaculture, in S. Simard (Ed.), Climate Change and Variability, (Europe: Sciyo, Intech, 2010) 1-486.
Effect of silver nanoparticles on energy metabolism in selected tissues…
54
[4]. M. J. A. Sarkar and M. M. Rashid, Pathogenicity of the bacterial isolate Aeromonas hydrophila to catfishes, carps and perch, Journal of Bangladesh Agricultural University 10(1), 2012, 157-161.
[5]. J.L. Torres, M. Sharif and A.T. Law, Identification and virulence screening of Aeromonas spp. isolated from healthy and epizootic ulcerative syndrome (EUS) infected fish, in R. Hirano, and I. Hanyu (Eds.), Proceedings of the Second Asian Fisheries Forum, (Asian Fisheries Society, Manila, Philippine, 1996) pp. 663-668.
[6]. H.A.C.C. Perera, and A. Pathiratne, Enhancement of immune responses in Indian carp, Catla catla, following adistration of levamisole by immersion, in M.G. Bondad-Reantaso, C.V. Mohan, M. Crumlish, and R.P. Subasinghe, (Eds.), Diseases in Asian Aquaculture VI. Fish Health Section, (Asian Fisheries Society, Manila, Philippines, 2008) pp 505.
[7]. B. Xavier Innocent, M. Syed Ali fathima and D. Dhanalakshmi , Studies on the immouostimulant activity of Coriandrum sativum and resistance to Aeromonas hydrophila in Catla catla, Journal of Applied Pharmaceutical Science 1 (07), 2011, 132-135.
[8]. B. Kaleeswaran, S. Ilavenil, S. Ravikumar, Changes in biochemical, histological and specific immune parameters in Catla catla (Ham.) by Cynodon dactylon (L.), Journal of King Saud University – Science 24, 2012, 139-152
[9]. B. Kaleeswaran, S. Ilavenil, S. Ravikumar, Enhancement of specific immune response in Catla catla (Ham.) Against Aeromonas hydrophila by the extract of Cynodon dactylon (L.), Hitek Journal of Biological Sciences and Bioengineering 1 (1), 2012, 1-15. [10]. T. Vandevelde, H. Renner, G. Schlamp, Silver, silver compounds, and silver alloys. Ullmann's Encylopedia of Industrial Chemistry. chapter 10. (New York, NY, USA: John Wiley & Sons, 2009)
[11]. J.S. Kim, E. Kuk, K.N. Yu, J.H. Kim, S.J. Park, H.I.Lee, S.H. Kim, S.J. Park, Y.H. Park, C.Y. Hwang, Y.K. Kim, Y.S. Lee, D.H. Jeong, M.H. Cho, Antimicrobial effects of silver nanopaticles. Nanomedicine: Nanotechnology Biology and Medicine. 3, 2007, 95-101.
[12]. M. Soltani, M. Ghodratnema, H. Ahari, H.A. Ebrahimzadeh Mousavi, M. Atee, F. Dastmalchi, J. Rahmanya, The inhibitory effect of silver nanoparticles on the bacterial fish pathogens Streptococcus iniae Lactococcus garvieae Yersinia ruckeri Aeromonas hydrophila, International Journal of Veterinary Research 3,2009, 137-142. [13]. B. Lee, C. N. Duong, J. Cho, J. Lee, K. Kim, Y. Seo, P. Kim, K. Choi, and J. Yoon, Toxicity of Citrate-Capped Silver Nanoparticles in Common Carp (Cyprinus carpio), Journal of Biomedicine and Biotechnology, 2012 (doi:10.1155/2012/262670) [14]. T.M. Scown, E.M. Santos, B.D. Johnston, B. Gaiser, M. Baalousha S. Mitov, J.R. Lead, V. Stone, T.F. Fernandes, M. Jepson et al., Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. Toxicological Sciences 115, 2010, 521–534.
[15]. M. A. Jones, M. D. Powell, J. A. Becker, and C. G. Carter, Effect of an acute necrotic bacterial gill infection and feed deprivation on the metabolic rate of Atlantic salmon Salmo salar, Disease of Aquatic Organisms 78, 2007, 29-36.