October - 2019 (Volume-9 ~ Issue-10 ~ Series-1)

Paper Type

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Research Paper

Title

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Antiglycation Activity and Radical Scavenging Activity of Leaves of Artocarpusheterophyllus

Country

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Sri Lanka

Authors

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J A H Erangika || S A Deraniyagala || C D Fernando

Page No.

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01-08

There is much interest in plant based medicine with antioxidant and anti-glycation properties. Formation of advanced glycation end products (AGEs) is a major cause of serious chronic diabetic complications. The objective of this study was to analyze the radical scavenging activity of different solvent extracts and the protein glycation inhibitory potential on fructose mediated non enzymatic glycation by the aqueous leaf extract of Artocarpusheterophyllus(ALEAH). The ALEAH (ripe and raw) was prepared separately according to the preparation of a "Kasaya" in Ayurvedic medicine. The freeze dried powder was used for the investigations. The leaves were soaked in methanol or ethanol and shaken, filtered evaporated in order to obtain the respective extracts..............

 

Key Words: Antiglycation activity, Artocarpusheterophyllus, radical scavenging activity

[1]. P. PRD, "In Vitro Antiglycation Activity of Some Medicinal Plants Used in Diabetes Mellitus," Med. Aromat. Plants,2(6), 2013, 5–7.
[2]. H. K. I. Perera, J. Poongunran, W. K. V. Kalpani Premadasa, and J. A. V. P. Jayasinghe, "In vitro antiglycation and hypoglycaemic effects of Syzygium cumini leaf extracts," J. Natl. Sci. Found. Sri Lanka, 46( 3), 2018, 281.
[3]. V. P. Singh, A. Bali, N. Singh, and A. S. Jaggi, "Singh, Bali, Singh, Jaggi - Korean J Phyiol Pharmacol - 2014,"18, 2014, 1–14.
[4]. A. Meeprom, W. Sompong, C. B. Chan, and S. Adisakwattana, "Isoferulic acid, a new anti-glycation agent, inhibits fructoseand glucose-mediated protein glycation in vitro," Molecules, 18, 6, 2013, 6439–6454.
[5]. P. Perera, "In vitro study on antiglycation activity, antioxidant activity and phenolic content of Osbeckia octandra L. leaf decoction," … Pharmacogn. …,2(4) ,2013, 198–201.

 

Paper Type

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Research Paper

Title

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Protective Media for Storage of L. Pasteur Rabies Virus Strain at Different Temperatures

Country

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Ukraine

Authors

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Viktoriia Varianytsia || Igor Vysekantsev

Page No.

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09-18

Here, we studied various preserving media compositions for long-term storage of L. Pasteur rabies virus vaccine strain at –20 and –80ºС in rabies products pharmaceutical manufacturing. Differences in strain infectious activity were established after storage at –80 and –20ºС. Infectious activity of the virus stored at –80ºС was higher than in the samples stored at –20ºС, the differences were statistically significant. During storage at these temperatures within a year (observation period), the highest safety of L. Pasteur rabies virus strain was provided with preserving media based on growth medium without additives and supplemented with sucrose, gelatin, glycerol and DMSO. Considering regulatory requirements and rabies products manufacturing conditions, protective media based on DMEM with 0.1% BSA, both without cryoprotectants and with the addition of 2.5-10% sucrose and storage at –80ºС were recommended for the long-term preservation of the studied rabies virus strain.

 

Key Words: cell culture, long-term storage, protective media, rabies virus, virus infectious activity, virus safety.

[1]. V.V. Burkova, I.P. Vysekantsev, A.A. Lavrik, Preservation of infectious activity of rabies virus industrial strains stored at various temperatures, Zhivye biokosnye sist, (9), 2014, 1–11.
[2]. F.X. Meslin, M.M. Kaplan, H. Koprowski, editors, Laboratory techniques in rabies. 4th ed. (Geneva: WHO; 1996. 446 p.)
[3]. WHO Expert Committee on Biological Standardization. Recommendations for inactivated rabies vaccine for human use produced in cell substrates and embryonated eggs. In: WHO Technical Report Series – 941. Fifty-sixth report. (Geneva: WHO, 2007) 83–132.
[4]. C.K. Gupta, J. Leszczynski, Gupta RK, Siber GR. Stabilization of respiratory syncytial virus (RSV) against thermal inactivation and freeze-thaw cycles for development and control of RSV vaccines and immune globulin, Vaccine 18(15), 1996, 1417–1420.
[5]. D. Lalošević, V. Lalošević, L.J. Lazarević-Ivanc, I. Knežević, BHK-21 cell culture rabies vaccine: immunogenicity of a candidate vaccine for humans, Dev. Biol. 131, 2008, 421–429.

 

Paper Type

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Research Paper

Title

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Appropriate Use of Restricted Antibiotics: Utilization, Bacterial Resistance and Impact of Clinical Interventions in Prescribing Practice – A Tertiary Care Hospital Population-Based Study

Country

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India

Authors

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Gayathiri Govindaraju || Vini Susan John || Rinju Alias || Noby Noble || Vijayakumar Arumugam || Uma Maheswari

Page No.

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19-25

Aim of the study: The study was designed to monitor the usage of Intravenous (IV), Intramuscular (IM), Intrathecal and Nebulized preparations of restricted antibiotics and the existing resistance pattern in a tertiary care hospital. It is important to know the resistance pattern as resistance among both gram positive and gram-negative organisms become a concern in treating the disease in critically ill patients. Materials and methods: A prospective interventional study was carried out in a 850 bedded tertiary care private corporate hospital over a period of six months (December 2017 – June 2018). All patients receiving IV, IM, Intrathecal and Nebulized restricted antibiotics in inpatient wards were included in this study. The cases were analyzed for appropriateness of the antibiotics chosen based on the dose, frequency, choice of drug for the specific site of infection..........

 

Key Words: Antibiogram, Bacterial resistance, Clinical intervention, Restricted antibiotic, Utilization pattern.

[1]. Thuong M, Shortgen F, Zazempa V, et al. Appropriate use of restricted antimicrobial agents in hospitals: the importance of empirical therapy and assisted re-evaluation. JAC.2000;46:501 – 08.
[2]. Ozkurt Z, Erol S, Kadanali A et al. Changes in antibiotic use, cost and consumption after an antibiotic restriction policy applied by infectious disease specialists. Jpn. J. Infect. Dis.2005;58:338-43,
[3]. Raymond P Daniel, Pelletier J Shawn, Crabtree D. Traves et al. Impact of rotating empirical antibiotic schedule on infectious mortality in an intensive care unit. Crit Care Med.2001;29(6):1101-1108.
[4]. WHO. Global Report on Surveillance: Antimicrobial Resistance. 2014; 1-7. www.who.int/iris/bitstream .
[5]. Carlet J. Antibiotic Resistance: Protecting antibiotics – the declaration of world alligance against antibiotic resistance. Indian J Crit Care Med.2014;18:643-45.

 

Paper Type

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Research Paper

Title

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In vitro study of the effect avocado leaf and cats whiskers extractcombination as inhibitor of urine crystalization

Country

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Indonesia

Authors

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Rini Madyastuti || Ietje Wientarsih || Setyo Widodo || Erni H Purwaningsih || Eva Harlina

Page No.

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26-31

Kidney stone or urolithiasis is presence of one or more calculi in urinary system. It is a disease with high recurrence both in human and small animal. Calcium oxalate is a major component of urolithiasis. The aim of this study is to evaluate the activity of combination extract Persea americana Mill and Orthosiphon aristatus (Blume)Miq. in dissolving calcium oxalate by In Vitro.METHODSThe varian combination was compared with commercial brand (group K(+)) using artificial urine as solvent. This experiment used 11 group with varian combination Persea americana and Orthosiphon aristatus (Blume)Miqextract. The measurement of calcium from the solution was used spectrophotometric atomic absorption (AAS).RESULTS AND DISCUSSION.The result showed that combination of extract Persea americana Mill. and Orthosiphon aristatus was effectively in dissolving calcium oxalate. From the data, the combination of the extract at rasio (1:4) (P8) and (4:1) (P9) had the potency as the inhibitor urolithiasis.

 

Key Words: Persea americana Mill, Orthosiphon aristatus, kidney stone, calcium oxalate, urolithiasis.

[1]. Abdelwahab SI, Mohan S, Elhassan MM, Al-Mekhlafi N, Adam Mariod A, Abdul AB, et al. 2011. Antiapoptotic and antioxidant properties of Orthosiphon stamineus Benth : Intervention in the Bcl-2-mediated apoptotic pathway. Evidence-based Complementary and Alternative Medicine. Article ID 156765 :1-11. doi:10.1155/2011/156765
[2]. Adha AC, Prasetyo BF, Madyastuti R. 2010. Pengaruh pemberian ekstrak etanol daun alpukat (Persea americana Mill.) terhadap aktivitas diuretik tikus putih jantan Sprague Dawley. [Skripsi]. Bogor ID : IPB
[3]. Adyana IK, Setiawan F, Insanu M. 2013. From etnopharmacology to clinical study of Orthosiphon Stamineus Benth. Int J Pharm Pharm Sci. vol 5, issue 3, 66–73.
[4]. Beghalia M, Ghalem S, Allali H, Belouatek A, Marouf A. 2014. Effect of herbal extracts of Tetraclinis articulata and Chamaerops humilis on calcium oxalate crystals in vitro. Gomal J Med Sci. 2007 ; 5:55-8.
[5]. Brown SA. 2017. Urolithiasis in small animals [internet][diacu pada 13 Februari 2017] tersedia dari http : // www. Merckmanual.com/urinary system/noninfectious disease of the urinary system in small animals/urolithiasis.